STABILITY OF RIBONUCLEIC-ACID DOUBLE-STRANDED HELICES
STABILITY OF RIBONUCLEIC-ACID DOUBLE-STRANDED HELICES
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DOI：
10.1016/0022-2836(74)90357-x
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
UHLENBECK, OC
中科院分区：
文献类型：
--
作者：
BORER, PN;DENGLER, B;UHLENBECK, OC
的hypochromicity，作为一个函数的温度为19个寡核糖核苷酸能够形成完美的碱基配对的双螺旋，是用来提取螺旋形成的热力学参数。通过全或无螺旋解链模型分析数据，该模型允许将ΔG、ΔH和ΔSof形成分配给10个可能的沃森-克里克碱基配对最近邻序列中的每一个。发现受阻稳定性对序列有显著的依赖性，并给出了已知序列的任何RNA双螺旋的Tm的预测公式。
The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔSof formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict theTmof any RNA double helix of known sequence.