Studies of double layer capacitance and electron transfer at a gold electrode exposed to protein solutions
Studies of double layer capacitance and electron transfer at a gold electrode exposed to protein solutions
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DOI：
10.1016/j.electacta.2004.03.034
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发表时间：
2004-09-30
影响因子：
6.6
通讯作者：
Wallace, GG
中科院分区：
文献类型：
--
作者：
Moulton, SE;Barisci, JN;Wallace, GG
用电化学阻抗谱（EIS）研究了蛋白质在磷酸盐缓冲液中的电化学行为。暴露于人血清白蛋白（HSA）和免疫球蛋白G（IG.G）的溶液导致金电极溶液界面处的双电层电容（C-dl）减小和电荷转移电阻（R-ct）增加。最大的电容降低两种蛋白质时，观察到曝光发生在或更积极的电极开路电位（OCP）。在相同条件下，与HSA相比，暴露于IG.G导致电容的更大降低。这些电容和电荷转移电阻的变化归因于暴露期间在电极表面上形成蛋白质层。(C)2004爱思唯尔有限公司保留所有权利。
Electrochemical impedance spectroscopy (EIS) was used to investigate the electrochemical behaviour of a gold electrode exposed to proteins prepared in phosphate buffer. Exposure to solutions of human serum albumin (HSA) and immunoglobulin G (Ig.G) resulted in a decrease of the double layer capacitance (C-dl) and an increase in the charge transfer resistance (R-ct) at the gold electrode solution interface. The greatest capacitance decrease for both proteins was observed when exposure occurred at or more positive to the electrode open circuit potential (OCP). Exposure to Ig.G resulted in a greater decrease in capacitance as compared to HSA under identical conditions. These capacitance and charge transfer resistance variations were attributed to the formation of a proteinaceous layer on the electrode surface during exposure. (C) 2004 Elsevier Ltd. All rights reserved.