Biochemical studies of the peroxidase-mediated oxidation of tyrosine to melanin: demonstration of the hydroxylation of tyrosine by plant and human peroxidases.
Biochemical studies of the peroxidase-mediated oxidation of tyrosine to melanin: demonstration of the hydroxylation of tyrosine by plant and human peroxidases.
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过氧化物酶介导的酪氨酸氧化为黑色素的生化研究：植物和人类过氧化物酶对酪氨酸羟基化的演示。
DOI：
10.1042/bj1240439
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
D. Epstein
中科院分区：
文献类型：
--
作者：
R. Patel;M. Okun;L. Edelstein;D. Epstein
在碱性条件下，通过离心除去pH，通过硫化氢对该化合物的作用回收多巴。我们的实验表明，过氧化物酶介导的酪氨酸合成黑色素比蘑菇酪氨酸酶介导的合成黑色素快得多，因此更难识别多巴作为中间体。在过氧化物酶介导的反应中，采取了以下措施来提高多巴的产率：(A)降低酶的浓度；(B)降低辅因子的浓度；(C)降低过氧化氢的浓度；(D)缩短反应时间；(E)使用还原剂(抗坏血酸)。
at alkaline pH is removed by centrifugation and dopa is recovered by the action of hydrogen sulphide on this compound. Our experiments indicated that the peroxidase-mediated synthesis of melanin from tyrosine is much more rapid than that mediated by mushroom tyrosinase, with resultant greater difficulty in identifying dopa as an intermediate. The following measures were taken to increase the yield of dopa in the peroxidase-mediated reaction: (a) lowering of the concentration of enzyme; (b) lowering of the concentration of cofactor; (c) lowering of the concentration of hydrogen peroxide; (d) shortening of the reaction time; (e) use of a reducing agent (ascorbate).