Transcriptomic Insights into Phenological Development and Cold Tolerance of Wheat Grown in the Field
Transcriptomic Insights into Phenological Development and Cold Tolerance of Wheat Grown in the Field
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DOI：
10.1104/pp.17.01311
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发表时间：
2018-03-01
期刊：
影响因子：
7.4
通讯作者：
Fowler, D. Brian
中科院分区：
文献类型：
--
作者：
Li, Qiang;Byrns, Brook;Fowler, D. Brian
禾本科植物的冷驯化和越冬是由复杂的环境调控基因表达决定的。然而，研究这些复杂的冷反应的研究大多是在受控环境中进行的，只考虑对单一环境变量的反应。在这项研究中，我们已经全面剖析了全球的转录反应，在田间生长的春小麦和冬小麦（普通小麦）基因型和近等基因系的VRN-A1等位基因交换冠。这种深入的分析揭示了影响耐寒性和物候发育的多种信号传导和相互作用途径，以优化植物生长和发育，为各种越冬胁迫做准备。在VRN-A1位点的遗传差异的调查表明，春化的要求保持较高水平的冷响应途径，而VRN-A1基因促进花发育。我们的研究结果还表明了遗传背景对冷和开花途径表达的影响。茎尖发育延迟和诱导耐冷性之间的联系反映在脱落酸依赖性和C重复结合因子途径的逐渐上调上。这是伴随着下调的关键基因参与分生组织的发展，随着秋季的进展。冬小麦和春小麦遗传背景差异表达基因的染色体定位显示，6A和6D染色体上的基因表达有明显的偏向，表明在基因组水平上的转录调控。这一发现增加了遗传级联和基因相互作用的复杂性，决定了小麦物候发育和耐冷性状的进化模式。
Cold acclimation and winter survival in cereal species is determined by complicated environmentally regulated gene expression. However, studies investigating these complex cold responses are mostly conducted in controlled environments that only consider the responses to single environmental variables. In this study, we have comprehensively profiled global transcriptional responses in crowns of field-grown spring and winter wheat (Triticum aestivum) genotypes and their near-isogenic lines with the VRN-A1 alleles swapped. This in-depth analysis revealed multiple signaling, interactive pathways that influence cold tolerance and phenological development to optimize plant growth and development in preparation for a wide range of over-winter stresses. Investigation of genetic differences at the VRN-A1 locus revealed that a vernalization requirement maintained a higher level of cold response pathways while VRN-A1 genetically promoted floral development. Our results also demonstrated the influence of genetic background on the expression of cold and flowering pathways. The link between delayed shoot apex development and the induction of cold tolerance was reflected by the gradual up-regulation of abscisic acid-dependent and C-REPEAT-BINDING FACTOR pathways. This was accompanied by the down-regulation of key genes involved in meristem development as the autumn progressed. The chromosome location of differentially expressed genes between the winter and spring wheat genetic backgrounds showed a striking pattern of biased gene expression on chromosomes 6A and 6D, indicating a transcriptional regulation at the genome level. This finding adds to the complexity of the genetic cascades and gene interactions that determine the evolutionary patterns of both phenological development and cold tolerance traits in wheat.