Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane
Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane
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DOI：
10.3390/ijms21093098
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发表时间：
2020-05-01
影响因子：
5.6
通讯作者：
Jung, Ole
中科院分区：
文献类型：
--
作者：
Barbeck, Mike;Kuehnel, Lennart;Jung, Ole
简介：生物可吸收胶原屏障膜用于防止软组织过早长入并允许骨再生。对于容量稳定适应症，仅可使用不可吸收合成材料。本研究研究了一种新的生物可吸收氢氟酸（HF）处理的镁（Mg）网片在天然胶原蛋白膜中的体积稳定情况。材料和方法：HF处理和未经处理的镁进行了比较，在直接和间接的细胞相容性试验。在体内，18只新西兰白色家兔接受每4个8 mm颅骨缺损，并分为4组：（a）HF处理的Mg网片/胶原膜，（B）未处理的Mg网片/胶原膜，（c）胶原膜和（d）假手术。6、12、18周后，采用放射学和组织学方法测量镁降解和骨再生。结果：经HF处理的Mg在体外表现出较高的细胞相容性。组织病理学上，HF-Mg防止气穴，并通过单核细胞吞噬作用降解长达12周。未经处理的镁显示部分显着更多的气体腔和纤维组织反应。骨再生在所有组之间没有显著差异。讨论和结论：嵌入天然胶原蛋白膜中的HF-Mg补片代表了不可吸收合成材料的体积稳定和生物相容性替代品。HF-Mg显示出较少的腐蚀性并且通过吞噬作用降解。然而，膜的应用并没有导致更高的骨再生。
Introduction: Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations. Materials and Methods: HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods. Results: In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups. Discussion and Conclusions: HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.