Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells.
Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells.
复制标题
DOI：
10.1038/srep20027
复制
发表时间：
2016-01-22
影响因子：
4.6
通讯作者：
Sone H
中科院分区：
文献类型：
--
作者：
Katsura M;Cyou-Nakamine H;Zen Q;Zen Y;Nansai H;Amagasa S;Kanki Y;Inoue T;Kaneki K;Taguchi A;Kobayashi M;Kaji T;Kodama T;Miyagawa K;Wada Y;Akimitsu N;Sone H
长期低剂量辐射对人类健康的影响尚未得到充分证实。最近的研究表明，神经前体细胞不仅存在于胎儿脑中，而且存在于成人脑中。由于未成熟的细胞通常更辐射敏感，在这里，我们研究了慢性低剂量辐射对培养的人神经祖细胞（hNPC）来自胚胎干细胞的影响。给予hNPC 31、124和496 mGy/72 h的低剂量辐射。通过微阵列分析的基因表达谱以及形态学分析来估计效果。基因表达量的辐射依赖性改变。通过31 mGy的辐射，涉及干扰素信号传导和细胞连接的炎症途径被改变。DNA修复和细胞粘附分子受到124 mGy辐射的影响，而DNA合成，凋亡，代谢和神经分化都受到496 mGy辐射的影响。这些体外结果表明，496 mGy辐射影响神经元祖细胞的发育，而在低于100 mGy的辐射剂量下观察到改变的基因表达。本研究将有助于阐明慢性低剂量辐射引起的神经元发育受损的临床和亚临床表型。
The effects of chronic low-dose radiation on human health have not been well established. Recent studies have revealed that neural progenitor cells are present not only in the fetal brain but also in the adult brain. Since immature cells are generally more radiosensitive, here we investigated the effects of chronic low-dose radiation on cultured human neural progenitor cells (hNPCs) derived from embryonic stem cells. Radiation at low doses of 31, 124 and 496 mGy per 72 h was administered to hNPCs. The effects were estimated by gene expression profiling with microarray analysis as well as morphological analysis. Gene expression was dose-dependently changed by radiation. By thirty-one mGy of radiation, inflammatory pathways involving interferon signaling and cell junctions were altered. DNA repair and cell adhesion molecules were affected by 124 mGy of radiation while DNA synthesis, apoptosis, metabolism, and neural differentiation were all affected by 496 mGy of radiation. These in vitro results suggest that 496 mGy radiation affects the development of neuronal progenitor cells while altered gene expression was observed at a radiation dose lower than 100 mGy. This study would contribute to the elucidation of the clinical and subclinical phenotypes of impaired neuronal development induced by chronic low-dose radiation.