Precipitation and Recovery of Cytochrome c and Hemoglobin Using AOT and Acetone
Precipitation and Recovery of Cytochrome c and Hemoglobin Using AOT and Acetone
复制标题
使用 AOT 和丙酮沉淀和回收细胞色素 c 和血红蛋白
DOI：
--
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
J. Vera
中科院分区：
文献类型：
--
作者：
Youn;E. Rodil;J. Vera
摘要利用磺基琥珀酸二（2-乙基己基）酯（AOT）作为沉淀剂，对细胞色素c和血红蛋白这两种转运蛋白进行了沉淀。当AOT与靶蛋白的摩尔比为11（细胞色素c）和30（血红蛋白）时，沉淀百分比为100%。以丙酮为极性溶剂，细胞色素c和血红蛋白的最大回收率分别为98%和40%。回收产物中的表面活性剂污染低于检测限。纯化1摩尔目标蛋白质所需的表面活性剂用量比使用AOT时反胶束提取所需的用量小许多个数量级。提出的沉淀法的优点，蛋白质纯化的反胶束萃取法进行了讨论。
Abstract The precipitation of two transport proteins, cytochrome c and hemoglobin, was carried out by using di‐(2‐ethylhexyl) sulfosuccinate, known as aerosol‐OT (AOT), as a precipitating agent. The percent precipitation was 100% when the molar ratio between AOT and the targeted protein was 11 for cytochrome c and 30 for hemoglobin. By using acetone as a polar solvent, the maximum recovery obtained was 98% for cytochrome c and 40% for hemoglobin. The surfactant contamination in the recovered product was below the detection limit. The required usage of surfactant to purify 1 mole of targeted protein was many orders of magnitude smaller than that required for a reverse micellar extraction when using AOT. Advantages of the suggested precipitation method over the reverse micellar extraction method for protein purification are discussed.