Microbial community dynamics in Baolige oilfield during MEOR treatment, revealed by Illumina MiSeq sequencing
Microbial community dynamics in Baolige oilfield during MEOR treatment, revealed by Illumina MiSeq sequencing
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DOI：
10.1007/s00253-015-7073-4
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发表时间：
2016-02
影响因子：
5
通讯作者：
Jing You;Gang Wu;Fuping Ren;Q. Chang;Bo Yu;Yanfen Xue;Bozhong Mu
中科院分区：
文献类型：
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作者：
Jing You;Gang Wu;Fuping Ren;Q. Chang;Bo Yu;Yanfen Xue;Bozhong Mu
本研究旨在了解微生物采油(MEOR)过程中微生物的多样性和作用，并评估MEOR处理对油藏微生物群落的影响。采用基于Illumina MiSeq的微生物群落结构与动态分析方法，对宝力格油田某区块微生物群落结构和动态进行了研究。结果表明，微生物多样性较高，分析样品中有23个类群。在所有被分析的样品中，变形杆菌、细菌、拟杆菌、热细菌和Eurya chaeota的丰度都相对较高。注入细菌和营养物质后，微生物群落的组成发生了有趣的变化。在MEOR处理期间，群落以已知的利用碳氢化合物的假单胞菌和不动杆菌为主。治疗后，随着时间的推移，这两个属的丰度逐渐减少，而甲烷杆菌科以及已知的合养单胞菌属、微囊藻属、硫杆菌属和产热菌属逐渐增加。优势微生物种群的变化表明，随着时间的推移，微生物群落的存在，以及碳氢化合物的降解和乙酸酯和丙酸盐的合养氧化为甲烷。这项工作有助于更好地了解油藏中的微生物过程，并有助于优化微生物采油技术。
This study was carried out to understand microbial diversity and function in the microbial enhanced oil recovery (MEOR) process and to assess the impact of MEOR treatment on the microbial community in an oil reservoir. The Illumina MiSeq-based method was used to investigate the structure and dynamics of the microbial community in a MEOR-treated block of the Baolige oilfield, China. The results showed that microbial diversity was high and that 23 phyla occurred in the analyzed samples. Proteobacteria, Firmicutes, Bacteroidetes, Thermotogae, and Euryarchaeota were present in relatively high abundance in all analyzed samples. Injection of bacteria and nutrients resulted in interesting changes in the composition of the microbial community. During MEOR treatment, the community was dominated by the known hydrocarbon-utilizing generaPseudomonasandAcinetobacter. After the treatment, the two genera decreased in abundance over time while Methanobacteriaceae, as well as known syntrophic genera such asSyntrophomonas,Pelotomaculum,Desulfotomaculum, andThermacetogeniumgradually increased. The change in dominant microbial populations indicated the presence of a succession of microbial communities over time, and the hydrocarbon degradation and syntrophic oxidation of acetate and propionate to methane in the MEOR-treated oilfield. This work contributes to a better understanding of microbial processes in oil reservoirs and helps to optimize MEOR technology.