DNA sequence, structure, and phylogenetic relationship of the small subunit rRNA coding region of mitochondrial DNA from Podospora anserina.
DNA sequence, structure, and phylogenetic relationship of the small subunit rRNA coding region of mitochondrial DNA from Podospora anserina.
复制标题
鹅足孢线粒体 DNA 的小亚基 rRNA 编码区的 DNA 序列、结构和系统发育关系。
DOI：
10.1007/bf02102481
复制
发表时间：
1989
影响因子：
3.9
通讯作者：
Sogin,ML
中科院分区：
文献类型：
--
作者：
Cummings,DJ;Domenico,JM;Nelson,J;Sogin,ML
DNA序列分析和S1图谱定位5'和3'末端表明，鹅足孢线粒体（mt）小亚基rRNA编码区长度为1980 bp。 mt rRNA 的类似编码区在玉米中为 1962 bp，在酿酒酵母中为 1686 bp，在哺乳动物中为 956 bp，而其在大肠杆菌中的对应编码区为 1542 bp。 TheP。 anserinamt 16S-like rRNA比来自E.的rRNA长400个碱基。大肠杆菌，但可以折叠成类似的二级结构。额外的碱基似乎聚集在特定位置，包括 5' 和 3' 末端的延伸。与来自几种生物体的 16S 样 RNA 的二级结构图进行比较，我们能够确定该基因的高度保守和可变区域。系统发育树的构建表明，该基因与其他线粒体基因归为一组，但正如预期的那样，与真菌线粒体基因最接近。
DNA sequence analysis and the localization of the 5′ and 3′ termini by S1 mapping have shown that the mitochondrial (mt) small subunit rRNA coding region fromPodospora anserinais 1980 bp in length. The analogous coding region for mt rRNA is 1962 bp in maize, 1686 bp inSaccharomyces cerevisiae, and 956 bp in mammals, whereas its counterpart inEscherichia coliis 1542 bp. TheP. anserinamt 16S-like rRNA is 400 bases longer than that fromE. coli, but can be folded into a similar secondary structure. The additional bases appear to be clustered at specific locations, including extensions at the 5′ and 3′ termini. Comparison with secondary structure diagrams of 16S-like RNAs from several organisms allowed us to specify highly conserved and variable regions of this gene. Phylogenetic tree construction indicated that this gene is grouped with other mitochondrial genes, but most closely, as expected, with the fungal mitochondrial genes.