A novel molecularly imprinted solid-phase microextraction fiber coupled with high performance liquid chromatography for analysis of trace estrogens in fishery samples
A novel molecularly imprinted solid-phase microextraction fiber coupled with high performance liquid chromatography for analysis of trace estrogens in fishery samples
复制标题
新型分子印迹固相微萃取纤维与高效液相色谱联用用于分析渔业样品中的痕量雌激素
DOI：
10.1016/j.talanta.2009.11.015
复制
发表时间：
2010-03-15
期刊：
影响因子：
6.1
通讯作者：
Li, Gongke
中科院分区：
文献类型：
--
作者：
Hu, Yuling;Wang, Yangyang;Li, Gongke
结合分子印迹和固相微萃取（SPME）技术提供了一个强大的样品制备工具，在选择性，简单性和灵活性。以17 β-雌二醇为模板分子，采用改进的多元共聚法制备了一种新型的分子印迹聚合物（MIP）涂层固相微萃取纤维。良好的热稳定性和化学稳定性萃取性能表明，与商业SPME纤维和对照聚合物相比，MIP涂层纤维对模板分子具有更强的亲和力。由于形状和结构的相容性，所获得的纤维还表现出对17 β-雌二醇的结构相关化合物的特异性选择性。如雌三醇、雌酮和17 α-乙炔基炔雌醇。因此可应用于与高效液相色谱联用的复杂样品中这些雌激素的同时测定。研究了影响提取的变量。MIP涂层的纤维证明了其提取渔业样品中的雌激素的效率。方法的检出限为0.98-2.39 μ g L-1，回收率分别为80.0- 83.6%和85.0-94.1%。(C)2009 Elsevier B V.保留所有权利。
The combination of molecular imprinting and solid-phase microextraction (SPME) technique provides a powerful sample preparation tool in terms of selectivity, simplicity, and flexibility. This paper reports a novel molecularly imprinted polymer (MIP) coated SPME fiber with 17 beta-estradiol as template by improved multiple co-polymerization method The obtained fiber exhibits excellent characteristics such as high porosity, good thermal and chemical stability Extraction performance shows that the MIP-coated fiber has stronger affinity to the template molecule as compared with the commercial SPME fibers and the control polymer-coated fiber without addition of template Owing to the shape and structural compatibility, the obtained fiber also demonstrated specific selectivity to the structural related compounds of 17 beta-estradiol. such as estriol, estrone and 17 alpha-ethynylestradiol. and thus can be applied to simultaneous determination of these estrogens from complex samples coupled with high performance liquid chromatography. The variables that influence extraction were investigated. The MIP-coated fiber demonstrated its efficiency for extraction of estrogens in fishery samples. The detection limits were in the range of 0.98-2.39 mu g L-1, and the recoveries were 80.0-83 6% and 85.0-94.1% for fish and shrimp tissue samples, respectively. (C) 2009 Elsevier B V. All rights reserved.