Cancer-Cell-Targeted Theranostic Cubosomes
Cancer-Cell-Targeted Theranostic Cubosomes
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DOI：
10.1021/la501332u
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发表时间：
2014-06-03
期刊：
影响因子：
3.9
通讯作者：
Murgia, Sergio
中科院分区：
文献类型：
--
作者：
Caltagirone, Claudia;Falchi, Angela Maria;Murgia, Sergio
这项工作致力于开发一种新型的基于脂质的（立方体）治疗诊断纳米颗粒，能够同时宿主喜树碱（一种有效的抗癌药物）和基于方酸的近红外发射荧光探针。此外，为了赋予这些纳米颗粒靶向能力，使用Pluronic F108和叶酸缀合的Pluronic F108以适当比例的混合物将它们分散。通过SAXS，DLS和cryo-TEM技术进行的物理化学表征，证明可以有效地制备这样的立方体的水分散体，而物理化学表征表明，这些纳米颗粒可用于体内成像目的。这些创新的纳米粒子在靶向癌细胞的上级能力，强调通过研究脂质滴的改变后，在HeLa细胞暴露于靶向和非靶向立方体诱导。
This work was devoted to the development of a new type of lipid-based (cubosome) theranostic nanoparticle able to simultaneously host camptothecin, a potent anticancer drug, and a squarain-based NIR-emitting fluorescent probe. Furthermore, to confer targeting abilities on these nanoparticles, they were dispersed using mixtures of Pluronic F108 and folate-conjugated Pluronic F108 in appropriate ratios. The physicochemical characterization, performed via SAXS, DLS, and cryo-TEM techniques, proved that aqueous dispersions of such cubosomes can be effectively prepared, while the photophysical characterization demonstrated that these nanoparticles may be used for in vivo imaging purposes. The superior ability of these innovative nanoparticles in targeting cancer cells was emphasized by investigating the lipid droplet alterations induced in He La cells upon exposure to targeted and nontargeted cubosomes.