Oxalurate induction of multiple URA3 transcripts in Saccharomyces cerevisiae.
Oxalurate induction of multiple URA3 transcripts in Saccharomyces cerevisiae.
复制标题
酿酒酵母中多个 URA3 转录物的草酸诱导。
DOI：
10.1128/mcb.3.11.1889-1897.1983
复制
发表时间：
1983
影响因子：
5.3
通讯作者：
Cooper,TG
中科院分区：
文献类型：
--
作者：
Buckholz,RG;Cooper,TG
酵母URA 3基因定位于一个1.1kb的DNA片段上。用此片段作杂交探针，我们发现尿囊素降解系统的一种免费诱导剂-这种反应仅限于尿酸盐;其他引起尿囊素降解酶高水平合成的条件不会产生这种效果。两类RNA（1.0和1.5 kb）被发现是受尿酸诱导的。这两类都由URA 3基因编码，重叠，并且在它们的5′末端可能没有显著差异。北方印迹分析表明，1.5 kb转录本可能由1.0 kb转录本3′端下游延伸至0.5 kb的序列编码。通过S1核酸酶图谱分析1.0-kbURA 3主要转录物的终点，发现存在两个5′端，间隔5 - 10个核苷酸，和7个3′端，每个间隔5 - 20个核苷酸，在大约70个碱基的范围内。
TheURA3gene fromSaccharomyces cerevisiaeis localized on a 1.1-kilobase (kb) DNA fragment. By using this fragment as a hybridization probe, we found that oxalurate, a gratuitous inducer of the allantoin degradative system, also serves to induceURA3specific RNA. This response is restricted to oxalurate; other conditions which bring about high-level synthesis of the allantoin degradative enzymes did not produce the effect. Two classes of RNA (1.0 and 1.5 kb) were found to be oxalurate induced. Both classes are encoded by theURA3gene, overlap, and probably do not significantly differ at their 5′ termini. Northern blot mapping of the transcripts indicated that the 1.5-kb transcript was likely encoded by sequences extending up to 0.5 kb downstream from the 3′ terminus of the 1.0-kb transcript. Analysis of the endpoints of the major 1.0-kbURA3transcript by S1 nuclease mapping revealed the existence of two 5′ termini, separated by 5 to 10 nucleotides, and seven 3′ termini, separated by 5 to 20 nucleotides each, over a range of about 70 bases.