Overexpression of EiKCS confers paraquat-resistance in rice (Oryza sativa L.) by promoting the polyamine pathway.
Overexpression of EiKCS confers paraquat-resistance in rice (Oryza sativa L.) by promoting the polyamine pathway.
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EiKCS 的过度表达通过促进多胺途径赋予水稻 (Oryza sativa L.) 百草枯抗性。
DOI：
10.1002/ps.6628
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发表时间：
2022-01
影响因子：
4.1
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Luo, Qiyu;Chen, Shu;Zhu, Jiazheng;Ye, Laihua;Hall, Nathan Daniel;Basak, Suma;McElroy, Joseph Scott;Chen, Yong
百草枯是一种广泛使用的联吡啶类除草剂，它能产生活性氧，导致细胞死亡。随着越来越多的百草枯抗性杂草，百草枯抗性在植物中的机制仍然不清楚。本研究通过基因工程在水稻中的单个过表达系中验证了先前确认的百草枯抗性基因EiKCS的功能。过量表达EiKCS提高了转基因水稻（KCSox）的百草枯抗性。预施外源亚精胺（1.5 mmol L−1）（12 h）可减轻百草枯对水稻的伤害。百草枯在KCSox中诱导的伤害为19.57%，低于其在野生型（WT）水稻中诱导的伤害的32.22%。百草枯抗性机制是通过抗氧化酶活性的增加和内源性多胺的过量产生。KCSox中的精胺含量超过30 μg mL−1，而WT水稻中的精胺含量低于5 μg mL−1。定量蛋白质组学研究表明，转基因EiKCS基因编码的β-酮脂酰辅酶A（CoA）合酶（51.81倍）促进了多胺途径相关蛋白的合成。精氨酸脱羧酶（ADC）途径促进腐胺的合成。亚精胺合酶I（1.10倍）和三个eceriferum辅因子（CER）对百草枯胁迫有反应。我们在本研究中验证了腐胺（C18 H20 N2 O2）、亚精胺（C28 H31 N3 O3）和精胺（C38 H42 N4 O 4）。 EiKCS编码的牛筋草β-酮脂酰辅酶A合酶基因是转基因作物的理想候选基因，因为它的过量表达不仅能带来百草枯抗性，而且在不降低产量和稻米品质的情况下具有潜在的益处。版权所有© 2021作者。由John Wiley & Sons有限公司代表化学工业协会出版的《害虫管理科学》。新的EiKCS基因的过表达已被证明在水稻中赋予百草枯抗性。
Paraquat is used widely as one of the bipyridine herbicides, which generates reactive oxygen species to cause cell death. With a growing number of paraquat‐resistant weeds, the mechanism of paraquat‐resistance in plants remains unclear. This research verified the functions of a previously confirmed putative paraquat‐resistant gene, EiKCS, from paraquat‐resistant goosegrass by genetic engineering in a single overexpressing line in rice. Overexpression of EiKCS improved paraquat resistance in transgenic rice (KCSox). Pre‐applied (12 h) exogenous spermidine (1.5 mmol L−1), alleviated the injury of paraquat in rice. Paraquat induced injury in KCSox was 19.57%, which was lower than 32.22% injury it induced in wild‐type (WT) rice. The paraquat‐resistant mechanism was through the increased activity of antioxidant enzymes and the overproduction of endogenous polyamines. The spermine content in KCSox was more than 30 μg mL−1, while that in WT rice was less than 5 μg mL−1. Quantitative proteomics showed that β‐ketoacyl‐coenzyme A (CoA) synthase (51.81 folds) encoded by the transgenic EiKCS gene promoted the synthesis of the proteins involved with the polyamine pathway. The synthesized putrescine was promoted by the arginine decarboxylase (ADC) pathway. The spermidine synthase I (1.10‐fold) and three eceriferum cofactors (CERs) were responsive to the paraquat stress. We validated putrescine (C18H20N2O2) spermidine (C28H31N3O3), and spermine (C38H42N4O4) in this study. EiKCS encoding β‐ketoacyl‐CoA synthase from goosegrass has been shown as an ideal candidate gene for engineering genetically modified organism (GMO) crops, as its overexpression does not only bring paraquat‐resistance, but also have potential benefits without decreasing yield and rice grain quality. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. Overexpression of novel EiKCS gene, has been shown to confer paraquat resistance in rice.
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