Gene expression pattern of insect fat body cells from in vitro challenge to cell line establishment
Gene expression pattern of insect fat body cells from in vitro challenge to cell line establishment
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昆虫脂肪体细胞从体外激发到细胞系建立的基因表达模式
DOI：
10.1007/s11626-014-9798-2
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发表时间：
2014-09
影响因子：
2.1
通讯作者：
Qilian Qin
中科院分区：
文献类型：
--
作者：
Guiling Zhou;Ruihao Shu;Jihong Zhang;Qilian Qin
这些细胞系为在细胞和分子水平上理解生物现象的机制提供了极好的工具。新的细胞培养技术的不断发展是感兴趣的生物化学，免疫学和病毒学研究中使用。原代培养细胞的转化是建立昆虫细胞系的关键步骤，但对这些转化的分子基础知之甚少。在这里，我们发现，原代培养的细胞的细胞周期进程被延迟或停滞在G2/M的荧光激活细胞分选分析。本研究构建了甜菜夜蛾（Spodoptera exigua）永生相关基因的消减cDNA文库。基因本体和途径分析表明氧化磷酸化、PI 3 K-Akt信号通路和泛素蛋白酶体通路的成员参与导致细胞永生化的过程，值得进一步研究。我们的研究结果表明，肿瘤相关基因或这些途径的靶基因可能通过调节G2/M期细胞的进程，促进原代细胞的转化。
The cell lines provided excellent tools to understand the mechanism of biological phenomenon at the cellular and molecular levels. The continuous development of new cell culture technology is both of interest for use in biochemical, immunology, and virological studies. The transformation of cells of the primary culture is a key procedure for insect cell line establishment but little is known about the molecular basis of these changes. Here, we found that the cell cycle progression of the cells of the primary culture was delayed or arrested in G2/M by fluorescence-activated cell sorting analysis. In this study, two subtractive cDNA libraries were constructed to screen for immortal-related genes ofSpodoptera exigua(Lepidoptera: Noctuidae). Gene ontology and pathway analysis indicated that members of the oxidative phosphorylation, PI3K-Akt signaling pathway, and the ubiquitin proteasome pathway are involved in processes leading toward cell immortalization merit further investigation. Our findings suggest that tumor-related genes or target genes of these pathways may contribute to the transformation of primary cell through regulation of G2/M cell cycle progression.
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影响因子：
8.8
作者：
通讯作者：
--
DOI：
--
发表时间：
1998-07
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
--
作者：
P. Flatt;J. Price;A. Shaw;J. Pietenpol
通讯作者：
P. Flatt;J. Price;A. Shaw;J. Pietenpol
DOI：
10.1007/s11626-012-9511-2
发表时间：
2012-05
期刊：
In Vitro Cellular & Developmental Biology - Animal
影响因子：
--
作者：
Xuan Li;Q. Qin;Ning Zhang;Wei Zhu;Jihong Zhang;Hong-tuo Wang;L. Miao;Huan Zhang
通讯作者：
Xuan Li;Q. Qin;Ning Zhang;Wei Zhu;Jihong Zhang;Hong-tuo Wang;L. Miao;Huan Zhang
DOI：
10.3109/10409238.2011.575764
发表时间：
2011-08
影响因子：
6.5
作者：
Deckbar D;Jeggo PA;Löbrich M
通讯作者：
Löbrich M
影响因子：
8
作者：
Meyer, KM;Hess, SM;Leadon, SA
通讯作者：
Leadon, SA