Mass fragmentographic determination of ferulic acid in plasma after oral administration of gamma-oryzanol.
Mass fragmentographic determination of ferulic acid in plasma after oral administration of gamma-oryzanol.
复制标题
口服γ-谷维素后血浆中阿魏酸的质量碎片测定。
DOI：
10.1248/cpb.30.973
复制
发表时间：
1982
影响因子：
1.7
通讯作者：
N. Awata
中科院分区：
文献类型：
--
作者：
S. Fujiwara;K. Noumi;I. Sugimoto;N. Awata
建立了γ-谷维素主要代谢产物阿魏酸在家兔和犬血浆中的质谱分析方法。在通过Amberlite XAD-2色谱法和溶剂萃取进行分离后，将阿魏酸转化为七氟丁酰乙酯衍生物，并使用4-羟基-3-三氘甲氧基肉桂酸（阿魏酸-d3）作为内标进行分析。在5.0-150.0 ng/ml血浆范围内，血浆样品中阿魏酸的回收率为100.5±4.5%（平均值±标准差，n=20）。以25、50和100 mg/kg体重的γ-谷维素芝麻油溶液经口给予家兔和犬，然后用质谱法测定阿魏酸的血浆浓度。γ-谷维素的给药剂量与阿魏酸的药时曲线下面积（AUC）具有良好的相关性，因此阿魏酸的AUC可作为评价γ-谷维素胃肠道吸收程度的指标。
A mass fragmentographic method for the quantitative determination of ferulic acid, a main metabolite of γ-oryzanol in rabbit and dog plasma, was developed. After its isolation by Amberlite XAD-2 chromatography and by solvent extraction, ferulic acid was converted to the heptafluorobutyryl ethyl ester derivative and analyzed by using 4-hydroxy-3-trideuteromethoxycinnamic acid (ferulic acid-d3) as an internal standard. The recovery of ferulic acid from plasma samples was 100.5±4.5% (mean±standard deviation, n=20) in the range of 5.0-150.0 ng/ml plasma. γ-Oryzanol was administered orally to rabbits and dogs at doses of 25, 50 and 100 mg/kg body weight as a solution of sesame oil, and then the plasma concentrations of ferulic acid were determined by mass fragmentography. A good correlation between the administered dose of γ-oryzanol and the area under the concentration curve (AUC) of ferulic acid was obtained in both animals, so the AUC of ferulic acid may be used as an index for estimating the extent of gastrointestinal absorption of γ-oryzanol.