Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins
Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins
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DOI：
10.1046/j.1365-313x.1999.00502.x
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发表时间：
1999-07-01
期刊：
影响因子：
7.2
通讯作者：
Choi, G
中科院分区：
文献类型：
--
作者：
Johnson, ET;Yi, HK;Choi, G
一些被子植物被限制在一系列可能的花色。这种限制可能是由于缺乏花青素苷生物合成基因或关键花青素苷生物合成酶二氢黄酮醇4-还原酶（DFR）的底物特异性。大花蕙兰的花主要产生矢车菊素型（粉红色到红色）花青素，而缺乏天竺葵素型（橙子到砖红色）花青素。为了研究这种花色范围的潜在分子机制，我们克隆了一个兰属植物DFR基因，并将其转化到DFR-矮牵牛系中。我们发现，兰DFR没有有效地减少二氢山奈酚（DHK），这是一个重要的步骤，天竺葵素生产。系统发育分析的DFR序列的数量表明，不能催化DHK还原至少发生了两次被子植物进化。我们的研究结果表明，在大花蕙兰中开发天竺葵素型橙子花色可能需要转化能够有效催化DHK还原的DFR基因。
Some angiosperms are limited to a range of possible flower colors. This limitation can be due to the lack of an anthocyanin biosynthetic gene or to the substrate specificity of a key anthocyanin biosynthetic enzyme, dihydroflavonol 4-reductase (DFR). Cymbidium hybrida orchid flowers primarily produce cyanidin-type (pink to red) anthocyanins and lack the pelargonidin-type (orange to brick-red) anthocyanins. To investigate the underlying molecular mechanism of this flower color range, we cloned a Cymbidium DFR gene and transformed it into a DFR- petunia line. We found that the Cymbidium DFR did not efficiently reduce dihydrokaempferol (DHK), which is an essential step for pelargonidin production. Phylogenetic analysis of a number of DFR sequences indicate that the inability to catalyze DHK reduction has occurred at least twice during angiosperm evolution. Our results indicate that developing a pelargonidin-type orange flower color in Cymbidium may require the transformation of a DFR gene that can efficiently catalyze DHK reduction.