Object recognition test in mice
Object recognition test in mice
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DOI：
10.1038/nprot.2013.155
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发表时间：
2013-12-01
期刊：
影响因子：
14.8
通讯作者：
Freret, Thomas
中科院分区：
文献类型：
--
作者：
Leger, Marianne;Quiedeville, Anne;Freret, Thomas
物体识别测试现在是最常用的小鼠行为测试之一。在第一个会话期间向鼠标呈现两个类似的对象，然后在第二个会话期间用新对象替换这两个对象中的一个。探索新物体所花费的时间量提供了识别记忆的指标。随着越来越多的小组使用该协议，物体识别测试中使用的程序的可变性稳步增加。该方案提供了必要的程序标准化。该协议减少了个体间的变异性与使用的选择标准的基础上，在每个会话期间的最小时间的探索两个对象。在这个协议中，我们描述了三个最常用的变体，包含长（3天），短（1天）或没有习惯化阶段。因此，在短的会话间隔（e。例如，在一个实施例中，6 h），根据适应期的持续时间，该程序可分别在4、2或1 d内进行。该方案应允许对不同研究的结果进行比较，同时允许方案适应实验者的约束。
The object recognition test is now among the most commonly used behavioral tests for mice. A mouse is presented with two similar objects during the first session, and then one of the two objects is replaced by a new object during a second session. The amount of time taken to explore the new object provides an index of recognition memory. As more groups have used the protocol, the variability of the procedures used in the object recognition test has increased steadily. This protocol provides a necessary standardization of the procedure. This protocol reduces inter-individual variability with the use of a selection criterion based on a minimal time of exploration for both objects during each session. In this protocol, we describe the three most commonly used variants, containing long (3 d), short (1 d) or no habituation phases. Thus, with a short intersession interval (e. g., 6 h), this procedure can be performed in 4, 2 or 1 d, respectively, according to the duration of the habituation phase. This protocol should allow for the comparison of results from different studies, while permitting adaption of the protocol to the constraints of the experimenter.