Membrane-associated estrogen receptor and caveolin-1 are present in central nervous system myelin and oligodendrocyte plasma membranes
Membrane-associated estrogen receptor and caveolin-1 are present in central nervous system myelin and oligodendrocyte plasma membranes
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DOI：
10.1002/jnr.20017
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发表时间：
2004-03-01
影响因子：
4.2
通讯作者：
Boggs, JM
中科院分区：
文献类型：
--
作者：
Arvanitis, DN;Wang, HM;Boggs, JM
雌激素受体（ER）是配体调节转录因子超家族的一员，被认为主要定位于细胞核；然而，最近在各种细胞中发现了一种与膜相关的内质网，它可以启动快速的非基因组细胞信号转导事件。尽管在少突胶质细胞中检测到核形式，但髓磷脂中ER的存在尚未报道。我们使用二维（2D） PAGE、质谱分析、肽质量指纹图谱、1d和2D凝胶的Western blotting和共聚焦显微镜发现，与ERbeta相似的内质网存在于分离的中枢神经系统（CNS）髓磷脂、脊髓和脑切片的髓鞘以及少突胶质细胞质膜中。Caveolin-1也被证明存在于分离的中枢神经系统髓鞘和少突胶质细胞膜中，在那里它与内质网部分共定位。在Triton X-100提取髓磷脂后，内质网存在于不溶性低密度的富含糖鞘脂的部分，甚至更多地存在于含有小窝蛋白和细胞骨架元素的高密度部分，这表明内质网的膜形式可能与小窝蛋白或髓磷脂的径向成分有关。雌激素在少突胶质细胞质膜和髓鞘内的发现表明雌激素在髓鞘维持或功能中的潜在作用。(C) 2004 Wiley-Liss, Inc。
The estrogen receptor (ER) is a member of a superfamily of ligand-regulated transcription factors that were thought to localize primarily to the nucleus; however, a membrane-associated ER that can initiate rapid non-genomic cell-signaling events has been identified recently in various cells. The presence of the ER in myelin has not been reported although the nuclear form has been detected in oligodendrocytes. We have shown that an ER with similarities to ERbeta is present in isolated central nervous system (CNS) myelin, the myelin sheath in spinal cord and brain sections, and the oligodendrocyte plasma membrane using two-dimensional (2D) PAGE, mass spectrometry, peptide mass fingerprinting, Western blotting of 1 D and 2D gels, and confocal microscopy. Caveolin-1 was also shown to be present in isolated CNS myelin and oligodendrocyte plasma membranes, where it was partially colocalized with ER. After Triton X-100 extraction of myelin, the ER was present in an insoluble low-density glycosphingolipid-enriched fraction and even more in a higher density fraction also containing caveolin and cytoskeletal elements, suggesting that the membrane form of ER may be associated with caveolin or the radial component of myelin. The discovery of the ER in the oligodendrocyte plasma membrane and within the myelin sheath indicates a potential role for estrogen in myelin maintenance or functions. (C) 2004 Wiley-Liss, Inc.