Functional reconnection of severed mammalian spinal cord axons with polyethylene glycol
Functional reconnection of severed mammalian spinal cord axons with polyethylene glycol
复制标题
DOI：
10.1089/neu.1999.16.727
复制
发表时间：
1999-08-01
影响因子：
4.2
通讯作者：
Blight, AR
中科院分区：
文献类型：
--
作者：
Shi, RY;Borgens, RB;Blight, AR
我们描述了一种技术，使用水溶性聚合物聚乙二醇（PEG）重新连接的两段完全横断的哺乳动物脊髓轴突在几分钟内。这是通过将完全切断的离体豚鼠胸部白色物质条融合在双蔗糖间隙记录室中来完成的。（MW 1，400 - 3，500 d;类似于蒸馏水中的50重量%）通过微量移液器直接施加到该区域，并在2分钟内通过抽吸移除。通过立即恢复复合动作电位通过原始细胞的传导记录成功融合。横切和可变数量的融合轴突，其中通过高分辨率光学显微镜和通过细胞内荧光染料通过融合轴突的扩散显示解剖连续性恢复。这些数据支持以下结论：一些切断的和随后PEG融合的脊髓轴突都表现出恢复的解剖连续性，并且在生理上也有能力传导动作电位。这项工作增加了我们以前的证明，PEG应用可以立即修复严重压碎，而不是切断，脊髓白色的问题，并可能导致新的治疗急性创伤的中枢和外周神经系统。
We describe a technique using the water-soluble polymer polyethylene glycol (PEG) to reconnect the two segments of completely transected mammalian spinal axons within minutes. This was accomplished by fusing completely severed strips of isolated guinea pig thoracic white matter maintained in vitro in a double sucrose gap recording chamber, The faces of the severed segments were pressed together, and PEG (MW 1,400-3,500 d; similar to 50% by weight in distilled water) was applied directly to this region through a micropipette and removed by aspiration within 2 min. Successful fusion was documented by the immediate restored conduction of compound action potentials through the original transection and by the variable numbers of fused axons in which anatomical continuity was shown to be restored by high-resolution light microscopy and by the diffusion of intracellular fluorescent dyes through fused axons, These data support the conclusion that some severed and subsequently PEG-fused spinal axons both demonstrate restored anatomical continuity and also are physiologically competent to conduct action potentials. This work adds to our previous demonstration that PEG application can immediately repair severely crushed, rather than cut, spinal cord white matter, and may lead to novel treatments for acute trauma to the central and peripheral nervous systems.