Comparison of two sampling methods for the detection of Gram-positive and Gram-negative bacteria in the environment:: moistened swabs versus Rodac plates
Comparison of two sampling methods for the detection of Gram-positive and Gram-negative bacteria in the environment:: moistened swabs versus Rodac plates
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DOI：
10.1078/s1438-4639(04)70035-8
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发表时间：
2001-03-01
影响因子：
6
通讯作者：
Lütticken, R
中科院分区：
文献类型：
--
作者：
Lemmen, SW;Häfner, H;Lütticken, R
本研究的目的是评估湿拭子技术与 Rodac 板在无生命环境中检测革兰氏阳性菌和革兰氏阴性菌的效果。在 22 个月的时间里，对 190 名感染或定植 MRSA、VRE 或多重耐药革兰氏阴性菌的患者的环境依次进行了采样。使用任一方法均可在 54 个病房中的 33 个 (61.1%) 病房、706 个 (24.6%) 环境样本中的 174 个环境样本中检测到 MRSA 和 VRE。然而，在 136 个房间中的 42 个房间 (30.9%) 中发现了多重耐药革兰氏阴性细菌，而在 1827 个环境样本中发现了 89 个房间 (4.9%) 的频率非常低 (p < 0.0001)。拭子技术对革兰氏阳性球菌的敏感性为 54 % (94/174)，而 Rodac 板为 69.5 % (121/174)（[CI95 %]，47-61 % 与 62-76 %，P < 0.05）。相比之下，拭子技术对革兰氏阴性菌的敏感性为 74.2% (66/89)，而 Rodac 板的敏感性为 42.7% (38/89)（[CI95%]，64-83% 对 32-54%，p < 0.05）。 总之，革兰氏阳性球菌的环境污染比革兰氏阴性菌更容易被检测到。对于革兰氏阳性球菌的检测，Rodac 平板优于拭子技术；而革兰氏阴性杆菌可以通过拭子技术更频繁地检测到。这些结果被证明具有统计显着性。
The objective of this study was to evaluate the moistened swab technique vs. Rodac plates for detecting Gram-positive and Gram-negative bacteria in the inanimate environment.Over a period of 22 months, the environment of 190 patients infected or colonized with MRSA, VRE or multiresistant Gram-negative bacteria was sampled in turn. MRSA and VRE could be detected with either method in 33 out of 54 (61.1 %) patient rooms in 174 out of 706 (24.6 %) environmental samples. However, multiresistant Gram-negative bacteria were found in 42 out of 136 (30.9 %) rooms with a very low frequency of 89 out of 1827 (4.9 %) environmental samples (p < 0.0001). The sensitivity of the swab technique for Gram-positive cocci was 54 % (94/174) vs. 69.5 % (121/174) for the Rodac plates, ([CI95 %], 47-61 % vs. 62-76 %, P < 0.05). In contrast, the sensitivity of the swab technique for Gram-negative bacteria was 74.2 % (66/89) vs. 42.7 % (38/89) for the Rodac plates, ([CI95 %], 64-83 % vs. 32-54 %, p < 0.05).In conclusion, environmental contamination with Gram positive cocci is detected more often than with Gram-negative bacteria. For the detection of Gram-positive cocci, Rodac plates are superior to the swab technique; whereas Gram-negative rods can be detected more often by the swab technique. hll these results proved to be statistically significant.