D-MEF2 - A MADS BOX TRANSCRIPTION FACTOR EXPRESSED IN DIFFERENTIATING MESODERM AND MUSCLE-CELL LINEAGES DURING DROSOPHILA EMBRYOGENESIS
D-MEF2 - A MADS BOX TRANSCRIPTION FACTOR EXPRESSED IN DIFFERENTIATING MESODERM AND MUSCLE-CELL LINEAGES DURING DROSOPHILA EMBRYOGENESIS
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DOI：
10.1073/pnas.91.12.5662
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发表时间：
1994-06-07
影响因子：
11.1
通讯作者：
OLSON, EN
中科院分区：
文献类型：
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作者：
LILLY, B;GALEWSKY, S;OLSON, EN
肌细胞增强因子（MEF）2家族的转录因子已涉及在脊椎动物肌肉转录的调节。我们已经从果蝇中克隆了一种蛋白质，称为D-MEF 2，它与脊椎动物MEF 2蛋白质的MADS（MCM 1，Agamous，Deficiens和血清反应因子）结构域具有广泛的氨基酸同源性。D-mef 2基因的表达首先在果蝇胚胎发生过程中检测到的中胚层前体细胞内的躯体和内脏肌肉谱系的规格之前。D-mef 2的表达依赖于中胚层的决定因素扭曲和蜗牛，但独立于含有同源框的基因tinman，这是内脏肌肉和心脏发育所需的。D-mef 2的表达先于MyoD同源物鹦鹉螺的表达，并且与鹦鹉螺相反，D-mef 2似乎在所有体细胞和内脏肌细胞前体中表达。D-mef 2的时空表达模式提示其可能在中胚层向肌源性谱系的定向分化中起重要作用。
The myocyte enhancer factor (MEF) 2 family of transcription factors has been implicated in the regulation of muscle transcription in vertebrates. We have cloned a protein from Drosophila, termed D-MEF2, that shares extensive amino acid homology with the MADS (MCM1, Agamous, Deficiens, and serum-response factor) domains of the vertebrate MEF2 proteins. D-mef2 gene expression is first detected during Drosophila embryogenesis within mesodermal precursor cells prior to specification of the somatic and visceral muscle lineages. Expression of D-mef2 is dependent on the mesodermal determinants twist and snail but independent of the homeobox-containing gene tinman, which is required for visceral muscle and heart development. D-mef2 expression precedes that of the MyoD homologue, nautilus, and, in contrast to nautilus, D-mef2 appears to be expressed in all somatic and visceral muscle cell precursors. Its temporal and spatial expression patterns suggest that D-mef2 may play an important role in commitment of mesoderm to myogenic lineages.