[A simple determination method of bromide ion in plasma of methyl bromide workers by head space gas chromatography].
[A simple determination method of bromide ion in plasma of methyl bromide workers by head space gas chromatography].
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顶空气相色谱法测定溴甲烷作业人员血浆中溴离子的简便方法
DOI：
10.1539/joh1959.29.196
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
S. Ishizu
中科院分区：
文献类型：
--
作者：
Y. Yamano;I. Ito;N. Nagao;S. Ishizu
本文介绍了一种简便测定血浆中溴离子的改进方法。Kawai，Sugiyama等人（1985）对该方法进行了修改。在改进的方法中，将200微升血浆和800微升蒸馏水置于反应瓶中，向其中加入100微升硫酸二甲酯。立即密封反应瓶，置于85 ℃的培养箱中，振荡10分钟。反应结束时，使用气密注射器抽出1 ml顶空气体。然后将溴甲烷气体注入气相色谱仪，用F.I.D.测定溴甲烷。溴离子浓度在0.5 ~ 500 ppm范围内与溴甲烷峰面积呈良好的线性关系。向血浆样品中加入标准品溶液中0.5 - 500 ppm范围内的溴离子。经10次重复测定，相对标准偏差在2.8-3.6%之间，回收率在96%以上。并与分光光度法进行了比较。对10名甲基溴作业工人的血浆样品进行了测定，结果表明，两种方法的测定结果吻合良好（r = 0.9669）。本文对39名溴甲烷作业工人进行了溴离子浓度测定，并与100名非溴甲烷作业工人（对照组）进行了比较。（250字处删节）
An improved method for the simple determination of bromide ion in plasma is described. It was modified from the method by Kawai, Sugiyama et al. (1985). In the improved method, 200 microliters of plasma and 800 microliters of distilled water were placed in a reaction vial to which 100 microliters of dimethyl sulfate was added. The reaction vial was immediately sealed, placed in an incubator at 85 degrees C, and shaken for 10 min. At the end of the reaction, a gas-tight syringe was used to draw out the head space gas of 1 ml. The methyl bromide gas was then injected into a gas chromatograph and methyl bromide was determined with F.I.D. A good linear relationship was obtained between the peak area of methyl bromide and the concentration of bromide ion within the range of 0.5 to 500 ppm. Bromide ion in the range of 0.5 to 500 ppm in standard solution was added to the plasma samples. After 10 repeated measurements, the relative standard deviation was found to be in the range of 2.8-3.6% and recovery was over 96%. The analytical results of this method were compared with those obtained by spectrophotometry. The analytical results obtained by the two methods were in good agreement (r = 0.9669) in plasma samples of 10 methyl bromide workers. A study to determine bromide ion concentration in 39 methyl bromide workers was made and the results were compared with those observed in 100 workers (control group) not exposed to methyl bromide.(ABSTRACT TRUNCATED AT 250 WORDS)