Preoperative diagnosis of thyroid carcinomas by aspiration biopsy-reverse transcription-polymerase chain reaction (ABRP): a report of two cases.
Preoperative diagnosis of thyroid carcinomas by aspiration biopsy-reverse transcription-polymerase chain reaction (ABRP): a report of two cases.
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甲状腺癌术前抽吸活检-逆转录聚合酶链反应（ABRP）诊断：附2例报告。
DOI：
10.1507/endocrj.47.271
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发表时间：
2000
影响因子：
2
通讯作者：
N. Amino
中科院分区：
文献类型：
--
作者：
T. Takano;E. Shiba;H. Tada;H. Yoshimura;K. Yoshida;R. Doi;N. Amino
最近发展了一种基于分子基础的甲状腺乳头状癌和间变性癌术前诊断技术，通过逆转录-聚合酶链反应（RT-PCR）检测细针穿刺活检（FNAB）中的癌胚纤维连接蛋白（onfFN）信使RNA（mRNA）。我们报告两例甲状腺肿瘤，其中细胞学诊断不成功，但其中RT-PCR分析提供的信息，有助于确定肿瘤的生物学特征。1例因固定不良，穿刺活检细胞学（ABC）未能确诊，但RT-PCR检测到onfFN mRNA表达，提示乳头状癌细胞的存在。手术后，此肿瘤被诊断为乳头状癌的组织学检查。另1例经ABC法确诊为乳头状癌，但FNAB中未检测到onfFN mRNA。组织学检查诊断为滤泡性肿瘤。这些情况下，结合遗传学和细胞学的方法来检查FNAB的好处。
A preoperative molecular-based diagnostic technique for thyroid papillary and anaplastic carcinomas was recently developed to detect oncofetal fibronectin (onfFN) messenger RNA (mRNA) in fine needle aspiration biopsy (FNAB) by means of reverse transcription-polymerase chain reaction (RT-PCR). We report two cases of thyroid tumors, in which cytological diagnosis was not successful but in which RT-PCR analysis provided information that helped to identify the biological features of the tumors. One case could not be diagnosed by aspiration biopsy cytology (ABC) because of poor fixation; however, RT-PCR did detect the expression of onfFN mRNA, which suggested the existence of papillary carcinoma cells. After surgery, this tumor was diagnosed as a papillary carcinoma by histological examination. The other case was diagnosed as a papillary carcinoma by ABC, but onfFN mRNA was not detected in the FNAB by RT-PCR. The neoplasm was diagnosed as a follicular tumor by histological examination. These cases suggest the benefits of combining both genetic and cytological approaches to the examination of FNAB.
甲状腺未分化癌中癌胚纤连蛋白mRNA的表达
DOI：
--
发表时间：
2007
期刊：
Ipn J Clin Oncol 37
影响因子：
--
作者：
Takano;T;et. al.
通讯作者：
et. al.