Charging and aggregation behavior of silica particles in the presence of lysozymes
Charging and aggregation behavior of silica particles in the presence of lysozymes
复制标题
DOI：
10.1007/s00396-017-4226-2
复制
发表时间：
2018-01-01
影响因子：
2.4
通讯作者：
Kobayashi, Motoyoshi
中科院分区：
文献类型：
--
作者：
Huang, Yi;Yamaguchi, Atsushi;Kobayashi, Motoyoshi
为了深入了解在蛋白质的存在下的胶体稳定性，我们测量了电泳迁移率和聚集速率常数的二氧化硅颗粒涂覆有溶菌酶，和吸附量的溶菌酶上的二氧化硅。我们还研究了模型分析，这是根据Derjaguin，朗道，Verwey和Overbeek理论与电荷异质性的影响，讨论溶菌酶涂层二氧化硅的聚集。我们的研究结果表明，当溶菌酶包覆的二氧化硅在等电点附近时，溶菌酶增强了二氧化硅的聚集。当溶菌酶吸附量较低时，电荷补丁吸引力的作用促进了二氧化硅的聚集。电荷不均匀性的影响随吸附量的增加而减弱。我们的模型考虑了电荷不均匀性的影响，可以定性地捕捉到溶菌酶存在下二氧化硅聚集的趋势，但理论预测与实验结果之间也存在很大的定量差异。需要进一步改进，以描述现实的电荷异质性和溶菌酶的表面覆盖的二氧化硅上的效果。
To gain insight into the colloidal stability in the presence of proteins, we measured the electrophoretic mobility and aggregation rate constant of silica particles coated with lysozymes, and the adsorbed amount of lysozymes on the silica. We also examined model analyses, which are based on the Derjaguin, Landau, Verwey, and Overbeek theory with the effect of charge heterogeneity, to discuss the aggregation of lysozyme-coated silica. Our results show that lysozymes enhance the aggregation of silica when the lysozyme-coated silica is near the isoelectric point. When the adsorbed amount of lysozyme is low, the effect of charge-patch attractive force promotes the aggregation of silica. The effect of charge heterogeneity weakens with the increase of adsorbed amount of lysozyme. Our model which takes account of the effect of charge heterogeneity can capture the trend of the aggregation of silica in the presence of lysozyme qualitatively, but there are also large quantitative discrepancies between the theoretical prediction and experimental results. Further improvement is required to describe realistic charge heterogeneity and the effect of the surface coverage of lysozyme on the silica.