CL- AND K+ TRANSPORT IN HUMAN BILIARY CELL-LINES
CL- AND K+ TRANSPORT IN HUMAN BILIARY CELL-LINES
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DOI：
10.1016/0016-5085(93)90661-u
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发表时间：
1993-06-01
期刊：
影响因子：
29.4
通讯作者：
FITZ, JG
中科院分区：
文献类型：
--
作者：
BASAVAPPA, S;MIDDLETON, J;FITZ, JG
背景：促进计费分泌和吸收的细胞机制尚未完全确定。这些研究的目的是评价人胆管肿瘤Mz-ChA-1和Sk-ChA-1细胞系的膜离子转运特性。方法：在培养的细胞中，使用125 I和36 Cl流出率来评估膜阴离子渗透性，使用86 Rb流出率来评估K+渗透性。结果：使用在BALB/ Urd小鼠上生长的肿瘤切片进行形态学评价并检测囊性纤维化跨膜电导调节因子（CFTR），囊性纤维化基因的蛋白产物。小管结构发育有序，γ-谷氨酰转肽酶和CK-19染色细胞。CFTR（可能是Cl−通道）的免疫过氧化物酶染色也存在。离子霉素或毒胡萝卜素刺激细胞内Ca 2+增加，125 I、36 Cl和86 Rb的外排增加。125 I的外排大于36 Cl，Cl−通道阻断剂4，4 ′-diisothiocyanatostilbene-2，2 ′-disulfonic acid可抑制阴离子外排。增加5′-环磷酸腺苷使36 Cl的外排大于125 I，但对86 Rb的外排无影响。这两种细胞系具有布美他尼敏感的86 Rb摄取一致可能的Na+/K+/2Cl− cotransfer.Conclusions：这些人类细胞系保留了分化的胆管细胞的某些表型特征，并可能是有用的胆汁液和电解质运输的进一步调查。
Background:The cellular mechanisms which contribute to billing secretion and absorption are not fully defined. The purpose of these studies was to evaluate the membrane ion transport properties of Mz-ChA-1 and Sk-ChA-1 cell lines derived from human biliary tumors.Methods:In cultured cells,125I and36Cl efflux rates were used to assess membrane anion permeability, and86Rb efflux rates were used to assess K+permeability.Results:Sections of tumors grown on BALB/ Urd mice were used for morphological evaluation and for detection of cystic fibrosis transmembrane conductance regulator (CFTR), the protein product of the cystic fibrosis gene. There was organized development of ductular structures and cells stained for γ-glutamyl transpeptidase and CK-19. Immunoperoxidase staining for CFTR, which is likely a Cl−channel, was also present. Increases in intracellular Ca2+stimulated by exposure to ionomycin or thapsigargin increased efflux of125I,36Cl, and86Rb. Efflux of125I was greater than36Cl, and anion efflux was inhibited by the Cl−channel blocker 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid. Increases in 5′-cyclic adenosine monophosphate increased efflux of36Cl greater than125I but had no effect on86Rb efflux. Both cell lines possess bumetanide-sensitive86Rb uptake consistent with possible Na+/K+/2Cl−cotransport.Conclusions:These human cell lines retain certain phenotypic features of differentiated biliary cells and may be useful for further investigation of biliary fluid and electrolyte transport.