Superporous agarose scaffolds for encapsulation of adult human islets and human stem-cell-derived β cells for intravascular bioartificial pancreas applications.
Superporous agarose scaffolds for encapsulation of adult human islets and human stem-cell-derived β cells for intravascular bioartificial pancreas applications.
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用于封装成人胰岛和人类干细胞衍生的 β 细胞的超孔琼脂糖支架，用于血管内生物人工胰腺应用。
DOI：
10.1002/jbm.a.37236
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Roy,Shuvo
中科院分区：
文献类型：
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作者：
Shaheen,Rebecca;Gurlin,RachelE;Gologorsky,Rebecca;Blaha,Charles;Munnangi,Pujita;Santandreu,Ana;Torres,Alonso;Carnese,Phichitpol;Nair,GopikaG;Szot,Gregory;Fissell,WilliamH;Hebrok,Matthias;Roy,Shuvo
伴有严重低血糖的1型糖尿病患者可以从细胞治疗中获益，如胰腺或胰岛移植；然而，供体短缺和免疫抑制的需要限制了广泛的临床应用。我们之前开发了一种血管内生物人工胰腺（iBAP），使用硅纳米孔膜（SNM）进行免疫保护。为了保证充足的营养输送，iBAP需要一个具有高渗透性的细胞支架来提供机械支持，维持胰岛的活力和功能。在这里，我们研究了超多孔琼脂糖（SPA）作为iBAP中潜在的细胞支架的可行性。SPA的水力渗透性比SNM高66倍，并且与最近的胰岛的扩散距离短（<10 μm）。SPA还支持在对流系统中封装的人类胰岛和干细胞衍生的富集β -簇的短期功能，证明了高活力（>95%）和对动态葡萄糖刺激的双相胰岛素反应。这些发现表明SPA支架不会限制对流生物人工胰腺的营养输送，值得继续研究。
Type 1 diabetic patients with severe hypoglycemia unawareness have benefitted from cellular therapies, such as pancreas or islet transplantation; however, donor shortage and the need for immunosuppression limits widespread clinical application. We previously developed an intravascular bioartificial pancreas (iBAP) using silicon nanopore membranes (SNM) for immunoprotection. To ensure ample nutrient delivery, the iBAP will need a cell scaffold with high hydraulic permeability to provide mechanical support and maintain islet viability and function. Here, we examine the feasibility of superporous agarose (SPA) as a potential cell scaffold in the iBAP. SPA exhibits 66‐fold greater hydraulic permeability than the SNM along with a short (<10 μm) diffusion distance to the nearest islet. SPA also supports short‐term functionality of both encapsulated human islets and stem‐cell‐derived enriched β‐clusters in a convection‐based system, demonstrated by high viability (>95%) and biphasic insulin responses to dynamic glucose stimulus. These findings suggest that the SPA scaffold will not limit nutrient delivery in a convection‐based bioartificial pancreas and merits continued investigation.
DOI：
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发表时间：
2003
期刊：
影响因子：
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作者：
P. Gustavsson;P. Larsson
通讯作者：
P. Larsson
超孔琼脂糖凝胶：生产、性能和应用
DOI：
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发表时间：
2009
期刊：
影响因子：
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作者：
P. Gustavsson;Peter Tiainen;P. Larsson
通讯作者：
P. Larsson