The effects of purine nucleotides on transmission in vesical parasympathetic ganglia of the cat.
The effects of purine nucleotides on transmission in vesical parasympathetic ganglia of the cat.
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DOI：
10.1111/j.1474-8673.1989.tb00208.x
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发表时间：
1989-06
期刊：
影响因子：
--
通讯作者：
R. Theobald;W. D. Groat
中科院分区：
文献类型：
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作者：
R. Theobald;W. D. Groat
1.在成年猫中，分离膀胱表面的节后神经纤维，并记录这些纤维的多单位活动。在这些猫中，膀胱插管，并记录膀胱内压。2. ATP，APPCP，ADP，AMP和腺苷抑制膀胱副交感神经节中的传输等效力，但是，2-氯腺苷是10倍以上的ATP。3. 2-氯腺苷、ATP、ADP、AMP、腺苷和APPCP抑制神经诱发的膀胱收缩的顺序或效力与它们抑制盆腔神经节传递的顺序或效力相同;然而，腺嘌呤、肌苷、IMP和ITP无效。4. 3'，5'-环腺苷酸和二丁酰环腺苷酸对膀胱活动几乎没有影响。5. ATP和APPCP在抑制所需剂量的2至50倍剂量下产生膀胱内压的瞬时升高，推测是通过ATP（P2）受体。APPCP对膀胱的兴奋作用是ATP的10至20倍。6.茶碱和咖啡因可有效拮抗盆神经节和膀胱平滑肌上腺苷（P1）受体介导的嘌呤能效应。7. ATP抑制TMA诱发的膀胱收缩和节后神经放电表明，嘌呤能抑制发生，至少部分，在神经节中的突触后位点。
1. In adult cats, postganglionic nerve fibres on the surface of the bladder were isolated and multiunit activity of these fibres was recorded. In these cats, the urinary bladder was cannulated and intravesical pressure was also recorded. 2. ATP, APPCP, ADP, AMP and adenosine depress transmission in vesical parasympathetic ganglia equipotently; however, 2-chloroadenosine was 10-fold more potent than ATP. 3. 2-chloroadenosine, ATP, ADP, AMP, adenosine and APPCP inhibit neurally evoked bladder contractions in the same order or potency with which they depress pelvic ganglionic transmission; however, adenine, inosine, IMP and ITP were ineffective. 4. 3',5'-cyclic AMP and dibutyryl cAMP produced little or no effect on bladder activity. 5. ATP and APPCP produced a transient rise in intravesical pressure at doses 2 to 50 times the dose needed for inhibition, presumably through ATP (P2) receptors. APPCP was 10 to 20 times more potent in exciting the bladder than ATP. 6. Theophylline and caffeine effectively antagonized purinergic effects mediated through adenosine (P1) receptors on both pelvic ganglia and bladder smooth muscle. 7. ATP inhibition of TMA-evoked bladder contractions and postganglionic nerve firing suggests that purinergic inhibition occurs, at least in part, at a postsynaptic site in the ganglia.