The heat shock protein 70 gene is involved for colony morphology, sporulation and mycoparasitism of Clonostachys rosea
The heat shock protein 70 gene is involved for colony morphology, sporulation and mycoparasitism of Clonostachys rosea
复制标题
热休克蛋白70基因与红竹的菌落形态、孢子形成和真菌寄生有关
DOI：
10.1093/femsle/fnz188
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发表时间：
2019-08-01
影响因子：
2.1
通讯作者：
Li, Shi-Dong
中科院分区：
文献类型：
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作者：
Sun, Zhan-Bin;Wang, Qi;Li, Shi-Dong
热休克蛋白70 （HSP70）是一种进化保守的伴侣蛋白。然而，HSP70在真菌寄生中的作用尚不清楚。牡丹对植物真菌病原菌具有很强的拮抗潜力。一种HSP70编码基因，crhsp，来自c。玫瑰67-1在寄生于菌核菌菌核过程中表达量显著上调。在本研究中，我们通过基因敲除实验研究了crhsp在支寄生虫病中的作用。结果表明，脆皮的破坏对玫瑰花的形态特征有显著的影响。此外，Lambda crhsp突变体在温室内对大豆菌核病的寄生能力和防治能力显著降低。结果表明，crcrsp参与了玫瑰镰刀菌的感染，为进一步研究玫瑰镰刀菌感染的分子机制提供了依据。这是首次报道HSP70基因参与玫瑰葡萄球菌支寄生虫病。
Heat shock protein 70 (HSP70) is an evolutionarily conserved chaperone protein. However, the role of HSP70 in mycoparasitism is unclear. Clonostachys rosea shows great potential against plant fungal pathogens. An HSP70 encoding gene, crhsp, fromC. rosea 67-1 was significantly upregulated during C. rosea parasitization of the sclerotia of Sclerotinia sclerotiorum. In the present study, we investigated the role of crhsp in mycoparasitism using gene knockout experiments. The results showed that disruption of crhsp had remarkabe effects on the morphological characteristics of C. rosea. In addition, the ability of C. rosea to parasitize sclerotia and control soybean Sclerotinia stem rot in the greenhouse was significantly reduced in the Lambda crhsp mutant. The results indicated that crhsp is involved in C. rosea mycoparasitism and provide the basis for further study of the molecular mechanism of C. rosea mycoparasitism. This is the first report to demonstrate the involvement of the HSP70 gene in C. rosea mycoparasitism.