Recovery of microbial community in strongly alkaline bauxite residues after amending biomass residue
Recovery of microbial community in strongly alkaline bauxite residues after amending biomass residue
复制标题
生物质残渣改良后强碱性铝土矿残渣中微生物群落的恢复
DOI：
10.1016/j.ecoenv.2022.113281
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发表时间：
2022-02-03
影响因子：
6.8
通讯作者：
Liu, Aiju
中科院分区：
文献类型：
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作者：
Dong, Mengyang;Hu, Shuxiang;Liu, Aiju
采用系统发育分析法研究了玉米秸秆生物量添加对铝土矿废渣中微生物群落的影响。土壤的地球化学，物理和生物学特性的改善进行了评估，以确定控制微生物群落发展的主要因素BR。经过一年的培养，经改良的BR的盐度和结构逐渐得到改善，pH值从11.39下降到9.89，交换性钠含量从86.3%下降到35.2%，平均重径（MWD）由0.12 mm增加到0.38 mm。结果表明，微生物对不同碳水化合物的利用发生了显著变化，多样性指数H'（0.7-7.34）、U（2.16-3.14）和平均显色率（0.059-1.08）均有所提高。经过一年的室外培养，细菌的优势菌门逐渐从子囊菌门转移（85.64%）为子囊菌门（52.07%）和担子菌门（35.53%），而优势菌门已从放线菌门转移变形菌门（21.39%）、芽单胞菌门（12.72%）、放线菌门（14.87%）、变形菌门（23.53%）、酸杆菌门（14.37%）。尽管有这些变化，微生物多样性仍然较低的修正BR比自然土壤。进一步的冗余度分析表明，pH值是驱动细菌群落变化的主要因素，而聚集体是驱动真菌群落变化的主要因素。本研究表明，玉米秸秆生物量的改良通过改善土壤环境条件，使BR中的微生物群落由嗜盐菌群向产酸菌群转变。
The aim of this study was to characterize the effects of cornstalk biomass amendments on microbial communities in bauxite residues (BRs) by phylogenetic analysis. Improvements in soil geochemical, physical, and biological properties were assessed to identify the major factors controlling microbial community development in BRs. After one year of incubation, the salinity and structure of the amended BRs had gradually improved, with pH dropping from 11.39 to 9.89, the exchangeable sodium percentage (ESP) dropping from 86.3% to 35.2%, and the mean weight diameter (MWD) rising from 0.12 mm to 0.38 mm. Further analysis of community level physio-logical profiles (CLPP) showed that the microbial utilization of different carbohydrates had shifted significantly, in addition to increases in the diversity index H' (0.7-7.34), U (2.16-3.14), and the average well color development (0.059-1.08). Over the one-year outside incubation, the dominant fungal phyla in the BRs had shifted gradually from Ascomycota (85.64%) to Ascomycota (52.07%) and Basidiomycota (35.53%), while the dominant bacterial phyla had shifted from Actinobacteria (38.47%), Proteobacteria (21.39%), and Gemmatimonadetes (12.72%) to Actinobacteria (14.87%), Proteobacteria (23.53%), and Acidobacteria (14.37%). Despite these shifts, microbial diversity remained lower in the amended BRs than in the natural soil. Further redundancy analysis indicated that pH was the major factor driving shifts in the bacterial community, while aggregates were the major factor driving shifts in the fungal community. This study demonstrated that amendment with cornstalk biomass shifted the microbial community in the BRs from halophilic groups to acidogenic groups by improving the soil environmental conditions.