Adhesive, sustained-release, antibacterial, cytocompatible hydrogel-based nanofiber membrane assembled from polysaccharide hydrogels and functionalized nanofibers
Adhesive, sustained-release, antibacterial, cytocompatible hydrogel-based nanofiber membrane assembled from polysaccharide hydrogels and functionalized nanofibers
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DOI：
10.1007/s10570-022-04894-y
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发表时间：
2022-10
期刊：
影响因子：
5.7
通讯作者：
Mengshi Wang;Jiatai Gu;Yunna Hao;X. Qin;Yanping Yu;Hongnan Zhang
中科院分区：
文献类型：
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作者：
Mengshi Wang;Jiatai Gu;Yunna Hao;X. Qin;Yanping Yu;Hongnan Zhang
水凝胶是一种三维亲水性聚合物交联网络，在生物医学、医疗保健等领域备受关注，由于它们独特的保水性和与细胞外基质的结构相似性。然而，常规的合成聚合物水凝胶具有差的生物相容性、粘附性和生物降解性。多糖作为天然细胞外基质的主要成分，具有良好的生物相容性和可降解性，因此多糖水凝胶在生物医学和医疗保健领域具有广阔的应用前景。本文通过功能化纳米纤维膜与多糖水凝胶的复合，制备了具有粘附性、缓释性、抗菌性和生物相容性的新型水凝胶基膜。采用绿色溶剂静电纺丝技术，以无毒、生物活性的聚己内酯为基材，制备了负载聚赖氨酸和尿囊素的功能化聚赖氨酸膜。以壳寡糖和氧化葡甘聚糖为原料，通过动态席夫碱交联制备了生物相容性多糖水凝胶。动态Schiff碱键赋予水凝胶优异的自修复性能。结果表明，水凝胶复合膜兼具纤维膜和水凝胶的双重特性，对大肠杆菌和金黄色葡萄球菌的抗菌率分别为99.99%和99.99%; 10 h内尿囊素累积释放率为65.7%，具有良好的缓释特性;这种独特的复合结构在护肤品的应用中表现出积极的潜力，包括面膜，医用敷料和其他产品。
Hydrogels, three-dimensional hydrophilic polymer cross-linked networks, have attracted much attention in the fields of biomedicine, health care, etc., due to their unique water retention, and structural similarity to extracellular matrix. However, the conventional synthetic polymer hydrogels have poor biocompatibility, adhesion, and biodegradability. Polysaccharides as a major constituent of native extracellular matrix are biocompatible and degradable, so the polysaccharides hydrogels are outstanding candidates for biomedicine and health care applications. Herein, novel hydrogel-based nanofiber membranes with adhesive, sustained-release, antibacterial and biocompatible performance were developed by complexing functionalized nanofibers membranes and polysaccharide hydrogels. The functionalized nanofiber membranes loaded with poly-lysine and allantoin were obtained by green solvent electrospinning technology using non-toxic and biological polycaprolactone as the base material. Biocompatible polysaccharide hydrogels were prepared by dynamic Schiff base crosslinking of chitosan oligosaccharide and oxidized konjac glucomannan. The dynamic Schiff base bonds endow hydrogels with outstanding self-healing properties. Consequently, with the integrated features of fibrous membranes and hydrogels, hydrogel-based nanofiber membranes have outstanding antibacterial capability against bothEscherichia coli(99.99%) andStaphylococcus aureus(99.99%), sustained-release characteristic with 65.7% cumulative allantoin release during 10 h, and biocompatibility with at least 450% cell viability within 5 days. This unique composite structure exhibits positive potential in the application of skincare, including facial masks, medical dressings and other products.