Formation of abdominal adhesions is inhibited by antibodies to transforming growth factor-beta1.
Formation of abdominal adhesions is inhibited by antibodies to transforming growth factor-beta1.
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转化生长因子-β1 抗体可抑制腹部粘连的形成。
DOI：
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发表时间：
1996
影响因子：
2.2
通讯作者：
Bok Y. Lee
中科院分区：
文献类型：
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作者：
Paul A. Lucas;Paul A. Lucas;D. Warejcka;Henry E. Young;Bok Y. Lee;Bok Y. Lee
转化生长因子- β (tgf - β)是调节成纤维细胞炎症反应和细胞外基质生成的重要因子。这两个过程在腹部手术后纤维粘连的形成中是相互联系的。当间皮受到损伤时，产生纤维蛋白链，首先由炎症细胞填充，然后由分泌细胞外基质形成永久粘附的成纤维细胞填充。tgf - β促进单核细胞趋化和成纤维细胞生成细胞外基质。我们使用了一个大鼠腹腔粘连模型，在这个模型中，一个腹膜圈被切开，然后再缝合到位。2周后处死大鼠，对粘连进行评分。六组每组10只大鼠接受了这项手术。第一组为手术对照组。第二组在术后即刻及术后第1、2天注射生理盐水（对照）。采用相同的方案，以生理盐水为载体，其他四组大鼠分别给予非特异性IgG（150微克/天）、抗tgf - β（泛特异性，167微克/天）、抗tgf - β 1（67微克/天）或抗tgf - β 2（50微克/天）。注射抗tgf - β 1的大鼠黏附评分明显低于对照组（P < 0.05）。注射抗tgf - β 2或抗tgf - β（泛特异性）的大鼠与注射盐水的对照大鼠无显著差异。结果表明，单独降低tgf - β 1水平可有效预防腹腔粘连。
Transforming growth factor-beta (TGF-beta) is an important factor in regulating the inflammatory response and the production of extracellular matrix by fibroblasts. These two processes are linked in the formation of fibrous adhesions after abdominal surgery. When the mesothelium is injured a fibrin strand is produced which is populated first by inflammatory cells then by fibroblasts which secrete extracellular matrix forming a permanent adhesion. TGF-beta promotes both chemotaxis of monocytes and the production of extracellular matrix by fibroblasts. We have used a model of abdominal adhesions in rats in which a circle of peritoneum is dissected and then sutured into place again. After 2 weeks the rats are euthanized and the adhesions are scored. Six groups of 10 rats each underwent this surgery. Group I served as the operative control. Group II was treated with saline which was injected immediately after surgery and on Days 1 and 2 after surgery (vehicle control). Using the same protocol with saline as vehicle, the other four groups of rats were treated with nonspecific IgG (150 microgram per day), anti-TGF-beta (panspecific, 167 microgram per day), anti-TGF-beta1 (67 microgram per day), or anti-TGF-beta2 (50 microgram per day). The rats injected with anti-TGF-beta1 had significantly lower adhesion scores (P < 0.05) than the controls. Rats injected with anti-TGF-beta2 or anti-TGF-beta (panspecific) did not differ significantly from the control saline-injected rats. The results indicate that specifically reducing levels of TGF-beta1 alone can be effective in preventing abdominal adhesions.