The mechanism of cell death in Listeria monocytogenes-infected murine macrophages is distinct from apoptosis
The mechanism of cell death in Listeria monocytogenes-infected murine macrophages is distinct from apoptosis
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DOI：
10.1128/iai.65.10.4075-4081.1997
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发表时间：
1997-10-01
影响因子：
3.1
通讯作者：
Kaufmann, SHE
中科院分区：
文献类型：
--
作者：
Barsig, J;Kaufmann, SHE
各种致病菌的能力，生活在真核细胞激活一个凋亡程序在感染的宿主细胞，诱导细胞凋亡单核细胞增生李斯特菌在小鼠树突状细胞和肝细胞已被描述，在这里我们解决的问题，是否和如何病原体杀死巨噬细胞，其最重要的栖息地，采用几种互补的技术，旨在区分细胞凋亡和坏死，我们发现小鼠骨髓源性巨噬细胞（BMM）感染产溶血素（Hly）的L.与福氏志贺菌感染或胶毒素处理的阳性对照相比，该病原体未能引起巨噬细胞的凋亡形态、DNA片段化和表面膜联蛋白V结合。单核细胞增多症释放的白细胞介素-1 β（IL-1 β）的量低于福氏志贺菌感染的单核细胞增多症，表明携带L.我们得出结论，小鼠BMM在L.病原体可以通过利用“假健康”外观的细胞的可能性而受益于该特征，从而避免被其他吞噬细胞快速消除。
Various pathogenic bacteria with the capacity to live within eukaryotic cells activate an apoptotic program in infected host cells, Induction of apoptosis by Listeria monocytogenes in murine dendritic cells and hepatocytes has been described, Here we address the questions of whether and how the pathogen kills macrophages, its most important habitat, Employing several complementary techniques aimed at discriminating between apoptosis and necrosis, we show that murine bone marrow-derived macrophages (BMM) undergo delayed necrosis but not apoptosis when infected with listeriolysin (Hly)-producing L. monocytogenes, This pathogen failed to elicit apoptotic morphology, DNA fragmentation, and surface annexin V binding of macrophages, in contrast to Shigella flexneri infection or gliotoxin treatment, which were used as positive controls, Furthermore, macrophages infected with L. monocytogenes released lower quantities of interleukin-1 beta (IL-1 beta) than did Shigella flexneri-infected ones, indicating diminished or even absent activation of IL-1-converting enzyme in macrophages harboring L. monocytogenes, We conclude that murine BMM die by necrosis after several hours of cytoplasmic replication of L. monocytogenes, The pathogen may benefit from this feature by the possibility of taking advantage of cells of ''pseudo-healthy'' appearance, thus avoiding rapid elimination by other phagocytes.