ULTRAVIOLET-RADIATION INCREASES HIV-LONG TERMINAL REPEAT-DIRECTED EXPRESSION IN TRANSGENIC MICE
ULTRAVIOLET-RADIATION INCREASES HIV-LONG TERMINAL REPEAT-DIRECTED EXPRESSION IN TRANSGENIC MICE
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DOI：
10.1089/aid.1991.7.729
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发表时间：
1991-09-01
影响因子：
1.5
通讯作者：
MARTIN, MA
中科院分区：
文献类型：
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作者：
FRUCHT, DM;LAMPERTH, L;MARTIN, MA
对携带人类免疫缺陷病毒(HIV)长末端重复序列(LTR)/氯霉素乙酰转移酶(CAT)转基因的FVB/N小鼠进行了不同剂量的254 nm UV-C或312 nm UV-B照射。在最佳暴露时间，在UV-C暴露后24小时收集的耳朵标本中，观察到HIV-LTR定向表达增加20倍；UV-B暴露诱导表达增加4倍。对体内UV-C诱导动力学的研究表明，LTR引导的基因表达在照射后2小时开始增加，并在照射后第3天达到最大值(>；30倍诱导)。在检测UV-B激活动力学的实验中，受辐射的转基因动物耳朵中CAT活性的最大水平是未受辐射的转基因对照的五倍(第5天)。此外，在紫外线照射后，毛皮皮肤中没有诱导CAT活性；然而，在UV-B治疗之前通过剃毛可以诱导HIV-LTR定向表达的四倍增加。
Previously described FVB/N mice harboring a human immunodeficiency virus (HIV) long terminal repeat (LTR)/chloramphenicol acetyl transferase (CAT) transgene were treated with varying amounts of 254 nm UV-C radiation or 312 nm UV-B radiation. At optimal exposure periods, a 20-fold increase in HIV-LTR-directed expression was observed in ear specimens collected 24 h following UV-C exposure; a fourfold increase in expression was induced by UV-B exposure. Investigation of the kinetics of UV-C induction in vivo revealed that LTR-directed gene expression began to increase 2 hours after exposure and reached a maximum on Day 3 following exposure (> 30-fold induction). In experiments examining the kinetics of UV-B activation, the maximum level of CAT activity in the ears of irradiated transgenic animals was fivefold above levels in unirradiated transgenic controls (Day 5). Furthermore, CAT activity was not induced in fur-bearing skin following UV exposure; however, a fourfold increase in HIV-LTR-directed expression could be elicited when hair was removed by shaving prior to UV-B treatment.