Performance of computer vision in vivo flow cytometry with low fluorescence contrast.
Performance of computer vision in vivo flow cytometry with low fluorescence contrast.
复制标题
低荧光对比度体内计算机视觉流式细胞术的性能。
DOI：
10.1117/1.jbo.20.3.035005
复制
发表时间：
2015
影响因子：
3.5
通讯作者：
Niedre,Mark
中科院分区：
文献类型：
--
作者：
Markovic,Stacey;Li,Siyuan;Niedre,Mark
小动物血流中循环细胞的检测和计数在临床前生物医学研究的许多领域都很重要，包括癌症转移、免疫学和生殖医学。Opticalin活体流式细胞术（IVFC）代表一类技术，可以在不抽取血液样本的情况下无创、连续地计数循环细胞。我们最近开发了一种称为计算机视觉的体内流式细胞术（CV-IVFC）的技术，该技术使用高灵敏度荧光相机和自动计算机视觉算法来询问小鼠耳朵中相对较大的循环血量。我们检测到循环细胞的浓度低至。在目前的工作中，我们的特点是性能的CV-IVFC与低对比度的成像条件与（1）弱细胞荧光标记，使用细胞模拟荧光微球具有不同的亮度和（2）高背景组织自发荧光通过不同的自发荧光特性的光学幻影。我们的分析表明，CV-IVFC可以强大的跟踪和计数循环细胞至少50%的灵敏度，即使在两个数量级的对比度下降的条件下，比我们以前的体内工作。这些结果支持CV-IVFC在广泛的体内生物学模型中的重要潜在效用。
Detection and enumeration of circulating cells in the bloodstream of small animals are important in many areas of preclinical biomedical research, including cancer metastasis, immunology, and reproductive medicine. Opticalin vivoflow cytometry (IVFC) represents a class of technologies that allow noninvasive and continuous enumeration of circulating cells without drawing blood samples. We recently developed a technique termed computer visionin vivoflow cytometry (CV-IVFC) that uses a high-sensitivity fluorescence camera and an automated computer vision algorithm to interrogate relatively large circulating blood volumes in the ear of a mouse. We detected circulating cells at concentrations as low as. In the present work, we characterized the performance of CV-IVFC with low-contrast imaging conditions with (1) weak cell fluorescent labeling using cell-simulating fluorescent microspheres with varying brightness and (2) high background tissue autofluorescence by varying autofluorescence properties of optical phantoms. Our analysis indicates that CV-IVFC can robustly track and enumerate circulating cells with at least 50% sensitivity even in conditions with two orders of magnitude degraded contrast than our previousin vivowork. These results support the significant potential utility of CV-IVFC in a wide range ofin vivobiological models.