Effects of lysophosphatidic acid on human colon cancer cells and its mechanisms of action
Effects of lysophosphatidic acid on human colon cancer cells and its mechanisms of action
复制标题
溶血磷脂酸对人结肠癌细胞的影响及其作用机制。
DOI：
10.3748/wjg.15.4547
复制
发表时间：
2009-09-28
影响因子：
4.3
通讯作者：
Pan, Bo-Rong
中科院分区：
文献类型：
--
作者：
Sun, Hong;Ren, Juan;Pan, Bo-Rong
目的：研究溶血磷脂酸(LPA)对人结肠癌细胞株SW480增殖、粘附、迁移和凋亡的影响及其作用机制。方法：采用甲基四唑法检测细胞增殖能力。采用流式细胞术检测细胞凋亡。使用Boyden transwell迁移室测量细胞迁移。根据实验方案在96孔板中进行细胞粘附测定。结果：与对照组相比，LPA以剂量依赖性和时间依赖性方式显着刺激SW480细胞增殖（P < 0.05），而丝裂原激活蛋白激酶（MAPK）抑制剂PD98059显着阻断LPA对增殖的刺激作用。 LPA还以剂量依赖性方式显着刺激SW480细胞的粘附和迁移（P < 0.05）。 Rho激酶抑制剂Y-27632显着抑制LPA对粘附和迁移的上调作用（P < 0.05）。 LPA显着保护细胞免受化疗药物顺铂和5-FU诱导的细胞凋亡（P < 0.05），但磷酸肌醇3激酶（PI3K）抑制剂LY294002显着阻断LPA对细胞凋亡的保护作用。结论：LPA刺激SW480细胞的增殖、粘附和迁移，并防止细胞凋亡。 Ras/Raf-MAPK、G12/13-Rho-RhoA 和 PI3K-AKT/PKB 信号通路可能参与其中。 (C) 2009 WJG 出版社和百事登。版权所有。
AIM: To study the effects of lysophosphatidic acid (LPA) on proliferation, adhesion, migration, and apoptosis in the human colon cancer cell line, SW480, and its mechanisms of action.METHODS: Methyl tetrazolium assay was used to assess cell proliferation. Flow cytometry was employed to detect cell apoptosis. Cell migration was measured by using a Boyden transwell migration chamber. Cell adhesion assay was performed in 96-well plates according to protocol.RESULTS: LPA significantly stimulated SW480 cell proliferation in a dose-dependent and time-dependent manner compared with the control group (P < 0.05) while the mitogen-activated protein kinase (MAPK) inhibitor, PD98059, significantly blocked the LPA stimulation effect on proliferation. LPA also significantly stimulated adhesion and migration of SW480 cells in a dose-dependent manner (P < 0.05). Rho kinase inhibitor, Y-27632, significantly inhibited the up-regulatory effect of LPA on adhesion and migration (P < 0.05). LPA significantly protected cells from apoptosis induced by the chemotherapeutic drugs, cisplatin and 5-FU (P < 0.05), but the phosphoinositide 3-kinase (PI3K) inhibitor, LY294002, significantly blocked the protective effect of LPA on apoptosis.CONCLUSION: LPA stimulated proliferation, adhesion, migration of SW480 cells, and protected from apoptosis. The Ras/Raf-MAPK, G12/13-Rho-RhoA and PI3K-AKT/PKB signal pathways may be involved. (C) 2009 The WJG Press and Baishideng. All rights reserved.