Evaluation of 2'-deoxy-2'-flouro-5-methyl-1-beta-D-arabinofuranosyluracil as a potential gene imaging agent for HSV-tk expression in vivo.
Evaluation of 2'-deoxy-2'-flouro-5-methyl-1-beta-D-arabinofuranosyluracil as a potential gene imaging agent for HSV-tk expression in vivo.
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评估 2-脱氧-2-氟-5-甲基-1-β-D-阿拉伯呋喃糖尿嘧啶作为 HSV-tk 体内表达的潜在基因成像剂。
DOI：
10.1162/15353500200202100
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发表时间：
2002
影响因子：
2.8
通讯作者：
Conti,PeterS
中科院分区：
文献类型：
--
作者：
Alauddin,MianM;Shahinian,Atranik;Gordon,ErlindaM;Conti,PeterS
2′-Deoxy-2′-flouro-5-methyl-1-β-d-arabinofuranosyluracil (FMAU) has been evaluated in HT-29 cells as a potential positron emission tomography (PET) radiotracer for imagingHSV-tkgene expression in vivo. In vitro experiments demonstrate that the accumulation of [14C]-FMAU inHSV-tk-expressing cells is 2.4-fold (p< .02), 4.0-fold (p< .001), and 5.3-fold (p< .001) higher than the wild-type cells at 1, 3, and 5 hr, respectively. In vivo studies revealed that the tumor uptake inHSV-tk-expressing cells was 2.3-fold (p< .001), 3.0-fold (p< .001), and 5.5-fold (p< .001) higher than the control cells at 1, 2, and 5 hr, respectively. FMAU was found to be more sensitive compared to our earlier studies using 9-[(3-18F-fluoro-1-hydroxy-2-propoxy)methyl]-guanine ([18F]-FHPG) and 9-(4-[18F]-fluoro-3-hydroxy-methylbutyl)guanine ([18F]-FHBG) in the same cell lines, although, the specificity was less than FHBG. These results suggest that while FMAU labeled with PET isotopes may be useful for imagingHSV-tk-expressing tumors in vivo, multitracer studies across additional tumor models are necessary in order to identify an optimal PET radiotracer.
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DOI：
--
发表时间：
2001
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
M. Iyer;J. R. Barrio;M. Namavari;Eileen Bauer;N. Satyamurthy;Khoi Nguyen;T. Toyokuni;Michael E. Phelps;Harvey R. Herschman;S. Gambhir
通讯作者：
M. Iyer;J. R. Barrio;M. Namavari;Eileen Bauer;N. Satyamurthy;Khoi Nguyen;T. Toyokuni;Michael E. Phelps;Harvey R. Herschman;S. Gambhir
(E)-5-(2-[125I]碘乙烯基)-2-脱氧尿苷的2-取代类似物在用单纯疱疹1型胸苷激酶基因转导的肿瘤细胞中的合成和细胞摄取。
影响因子：
7.3
作者：
K. Morin;E. Atrazheva;E. Knaus;L. Wiebe
通讯作者：
L. Wiebe
基于正电子发射断层扫描的转基因表达成像，由体内复制条件性溶瘤单纯疱疹病毒 1 型突变载体介导。
DOI：
--
发表时间：
2001
期刊：
Cancer research.
影响因子：
--
作者：
Jacobs,A;Tjuvajev,JG;Dubrovin,M;Akhurst,T;Balatoni,J;Beattie,B;Joshi,R;Finn,R;Larson,SM;Herrlinger,U;Pechan,PA;Chiocca,EA;Breakefield,XO;Blasberg,RG
通讯作者：
Blasberg,RG
影响因子：
4.8
作者：
Fu, DX;Kobayashi, M;Lin, L
通讯作者：
Lin, L
影响因子：
1.8
作者：
Alauddin, MM;Conti, PS;Fissekis, JD
通讯作者：
Fissekis, JD