Impaired cell-mediated immunity in mice bearing healthy orthotopic corneal allografts.
Impaired cell-mediated immunity in mice bearing healthy orthotopic corneal allografts.
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DOI：
10.4049/jimmunol.150.5.1727
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发表时间：
1993-03
影响因子：
4.4
通讯作者：
Y. Sonoda;J. Streilein
中科院分区：
文献类型：
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作者：
Y. Sonoda;J. Streilein
原位移植的、组织不相容的角膜在健康条件下被幼稚的未处理的BALB/c小鼠接受延长的间隔（> 8周）的比例非常高。由于这些移植物形成眼前房的前表面，并且由于放置在前房中的同种异体抗原引起异常的、刻板的、全身性的免疫反应，称为前房相关免疫偏离，我们已经检查了接受角膜移植物的BALB/c小鼠以及拒绝角膜移植物的小鼠中的同种异体抗原特异性细胞介导的免疫状态。尝试通过s.c.注射与移植物遗传上相同的淋巴样细胞，仅在“排斥”小鼠中产生阳性反应;“接受”小鼠不能产生或显示DH。然后测定来自受体和排斥者的脾细胞抑制同种异体抗原特异性DH的局部过继转移的能力。结果发现，转移反应未能发展，如果脾细胞从受体小鼠被添加到接种物，而脾细胞从拒绝小鼠没有类似的抑制作用。在局部过继转移试验中，受体脾细胞介导的抑制被确定对长期角膜同种移植物上表达的同种抗原具有特异性。我们的结论是，当成年小鼠无限期接受原位角膜同种异体移植物时，移植成功与诱导同种异体抗原特异性前房相关免疫偏离相关。有人提出，抑制同种异体抗原特异性DH，这是接受移植物的小鼠的特征，在移植角膜的成功中起着至关重要的作用。
A very high proportion of orthotopically grafted, histoincompatible corneas are accepted in healthy condition for prolonged intervals (> 8 weeks) by naive untreated BALB/c mice. Because these grafts form the anterior surface of the anterior chamber of the eye and because alloantigens placed in the anterior chamber elicit a deviant, stereotypic, systemic immune response known as anterior chamber-associated immune deviation, we have examined the state of alloantigen-specific cell-mediated immunity in BALB/c mice with accepted corneal allografts, as well as mice that had rejected their corneal grafts. Attempts to induce alloantigen-specific delayed hypersensitivity (DH) by s.c. injections of lymphoid cells, genetically identical to the grafts, resulted in positive responses only in "rejector" mice; "acceptor" mice failed to develop or display DH. Spleens cells from acceptors and rejectors were then assayed for the ability to suppress local adoptive transfer of alloantigen-specific DH. It was found that transfer reactions failed to develop if spleen cells from acceptor mice were added to the inoculum, whereas spleen cells from rejected mice had no similar inhibitory effects. The suppression mediated by acceptor spleen cells in local adoptive transfer assays was determined to be specific for the alloantigens expressed on the long-standing corneal allografts. We conclude that, when orthotopic corneal allografts are accepted indefinitely by adult mice, graft success correlates with the induction of alloantigenically specific anterior chamber-associated immune deviation. It is proposed that suppression of alloantigen-specific DH, which is characteristic of mice with accepted grafts, plays a critical role in the success of grafted corneas.