Synthetic ion channel based on metal-organic polyhedra
Synthetic ion channel based on metal-organic polyhedra
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DOI：
10.1002/anie.200802240
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发表时间：
2008-01-01
影响因子：
16.6
通讯作者：
Kim, Kimoon
中科院分区：
文献类型：
--
作者：
Jung, Minseon;Kim, Hyunuk;Kim, Kimoon
材料和方法。所有试剂和溶剂均购自商业来源并且不经进一步纯化即使用。氯仿中的蛋黄L-α-磷脂酰胆碱（EYPC）购自Avanti Polar Lipids，Inc.。(USA)并储存在冰箱中。所有囊泡溶液都用购自Avestin，Inc.的手持式挤出机挤出。根据文献1合成MOP-18，并从乙腈和甲苯中重结晶。MOP-18的结构在浦项加速器实验室（光束线6C 1）使用同步辐射通过单晶X射线晶体学确认。用Shimadzu RF-5301 PC比率荧光计进行荧光实验。在Perkin-Elmer Spectrum GX FT-IR系统上记录FT-IR光谱。动态光散射（DLS）实验用具有He-Ne激光（λ= 632.8nm）的Otsuka ELS 8000进行。
Materials and Methods. All the reagents and solvents were purchased from commercial sources and used without further purification. Egg yolk L-α-phosphatidylcholine (EYPC) in chloroform was purchased from Avanti Polar Lipids, Inc.(USA) and stored in a refrigerator. All the vesicle solutions were extruded with a handheld extruder purchased from Avestin, Inc. MOP-18 was synthesized according to the literature1 and recrystallized from actonitrile and toluene. The structure of MOP-18 was confirmed by single-crystal X-ray crystallography using synchrotron radiation at Pohang Accelerator Laboratory (Beamline 6C1). Fluorescence experiments were carried out with a Shimadzu RF-5301PC ratiometric fluorimeter. FT-IR spectra were recorded on a Perkin-Elmer Spectrum GX FT-IR system. Dynamic light scattering (DLS) experiments were performed with an Otsuka ELS 8000 with a He-Ne laser (λ= 632.8 nm).