Multiscale Imaging of Brown Adipose Tissue in Living Mice/Rats with Fluorescent Polymer Dots
Multiscale Imaging of Brown Adipose Tissue in Living Mice/Rats with Fluorescent Polymer Dots
复制标题
使用荧光聚合物点对活体小鼠/大鼠的棕色脂肪组织进行多尺度成像
DOI：
10.1021/acsami.8b06094
复制
发表时间：
2018-06-20
影响因子：
9.5
通讯作者：
Xiong, Liqin
中科院分区：
文献类型：
--
作者：
Guo, Yixiao;Li, Yao;Xiong, Liqin
由于棕色脂肪组织（BAT）具有适应性生热能力，因此该组织已被确定为治疗肥胖、糖尿病和相关代谢紊乱的有希望的靶标。可视化 BAT 可能为病理学研究、药物筛选和疗效评估提供重要工具。由于目前核磁共振成像方法检测BAT的局限性，荧光成像在小动物大规模临床前研究中具有优势。在此，基于聚合物点作为荧光探针对小鼠进行快速 BAT 成像。早在静脉注射聚合物点后 5 分钟，就在肩胛间 BAT 和腋窝 BAT 中检测到广泛的荧光。此外，还可以识别腋窝和腹股沟白色脂肪组织 (WAT)。使用基于探针的共焦激光内窥镜成像技术监测啮齿动物体内聚合物点的实时体内行为，并通过组织学测定证实了 BAT 中的优先积累优于 WAT。而且，整个研究是在没有低温或药物刺激的情况下进行的。成像功效在细胞、组织学和全身水平上得到了验证，目前的结果表明荧光聚合物点可能是活体受试者中 BAT 可视化的有前途的工具。
Brown adipose tissue (BAT) has been identified as a promising target for the treatment of obesity, diabetes, and relevant metabolism disorders because of the adaptive thermogenesis ability of this tissue. Visualizing BAT may provide an essential tool for pathology study, drug screening, and efficacy evaluation. Owing to limitations of current nuclear and magnetic resonance imaging approaches for BAT detection, fluorescence imaging has advantages in large-scale preclinical research on small animals. Here, fast BAT imaging in mice is conducted based on polymer dots as fluorescent probes. As early as 5 min after the intravenous injection of polymer dots, extensive fluorescence is detected in the interscapular BAT and axillar BAT. In addition, axillar and inguinal white adipose tissues (WAT) are recognized. The real-time in vivo behavior of polymer dots in rodents is monitored using the probe-based confocal laser endomicroscopy imaging, and the preferred accumulation in BAT over WAT is confirmed by histological assays. Moreover, the whole study is conducted without a low temperature or pharmaceutical stimulation. The imaging efficacy is verified at the cellular, histological, and whole-body levels, and the present results indicate that fluorescent polymer dots may be a promising tool for the visualization of BAT in living subjects.