Characterization of Fatty Acid Composition in Bone Marrow Fluid From Postmenopausal Women: Modification After Hip Fracture.
Characterization of Fatty Acid Composition in Bone Marrow Fluid From Postmenopausal Women: Modification After Hip Fracture.
复制标题
绝经后妇女的骨髓液中脂肪酸组成的表征：髋部骨折后修饰。
DOI：
10.1002/jcb.25534
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发表时间：
2016-10
影响因子：
4
通讯作者：
Rodríguez JP
中科院分区：
文献类型：
--
作者：
Miranda M;Pino AM;Fuenzalida K;Rosen CJ;Seitz G;Rodríguez JP
骨髓脂肪组织（BMAT）与低骨量相关，尽管目前尚不清楚BMAT增加对骨骼维持的功能后果。BMAT可能在全身能量代谢中起作用，并且可能是邻近骨细胞的能量来源以及内分泌器官，将细胞因子、脂肪因子和游离脂肪酸释放到骨髓微环境中。本报告的目的是比较绝经后妇女（65至80岁）的骨髓上清液（BMSF）和血浆中的脂肪酸组成。BMSF是在旋转抽吸的骨髓样本后获得的;供体在双能X线吸收测定法后被分类为对照、骨质减少或骨质疏松。从人骨髓液和血浆中提取总脂质，转化为相应的甲酯，最后用气相色谱仪与质谱仪联用分析。结果表明，BMSF中的脂肪酸组成是动态的，与血浆不同，这意味着在局部产生的脂质的意义。与血浆相比，BMSF中的脂肪酸组成富含饱和脂肪酸，不饱和脂肪酸减少，但这种关系在髋部骨折的女性中发生了变化。另一方面，BMSF和骨密度之间没有关系。总之，BMSF的脂质组成与循环隔室不同，很可能反映了骨髓隔室的能量需求。
Bone marrow adipose tissue (BMAT) is associated with low bone mass, although the functional consequences for skeletal maintenance of increased BMAT are currently unclear. BMAT might have a role in systemic energy metabolism, and could be an energy source as well as an endocrine organ for neighboring bone cells, releasing cytokines, adipokines and free fatty acids into the bone marrow microenvironment. The aim of the present report was to compare the fatty acid composition in the bone marrow supernatant fluid (BMSF) and blood plasma of postmenopausal women women (65 to 80 years old). BMSF was obtained after spinning the aspirated bone marrow samples; donors were classified as control, osteopenic or osteoporotic after dual-energy X-ray absorptiometry. Total lipids from human bone marrow fluid and plasma were extracted, converted to the corresponding methyl esters, and finally analyzed by a gas chromatographer coupled with a mass spectrometer. Results showed that fatty acid composition in BMSF was dynamic and distinct from blood plasma, implying significance in the locally produced lipids. The fatty acid composition in the BMSF was enriched in saturated fatty acid and decreased in unsaturated fatty acids as compared to blood plasma, but this relationship switched in women who suffered a hip fracture. On the other hand, there was no relationship between BMSF and bone mineral density. In conclusion, lipid composition of BMSF is distinct from the circulatory compartment, most likely reflecting the energy needs of the marrow compartment.