VESICULAR GLUTAMATE TRANSPORTERS: SPATIO-TEMPORAL PLASTICITY FOLLOWING HEARING LOSS
VESICULAR GLUTAMATE TRANSPORTERS: SPATIO-TEMPORAL PLASTICITY FOLLOWING HEARING LOSS
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DOI：
10.1016/j.neuroscience.2010.12.059
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发表时间：
2011-03-31
期刊：
影响因子：
3.3
通讯作者：
Holt, A. G.
中科院分区：
文献类型：
--
作者：
Fyk-Kolodziej, B.;Shimano, T.;Holt, A. G.
耳聋大鼠与正常听力大鼠耳蜗核vGluT1和vGluT3的免疫细胞化学比较首次在耳蜗核背侧和腹侧发现vGluT3免疫染色，揭示了耳蜗损伤后vGluT1在耳蜗核定位的时空变化。在正常听力大鼠中，vGluT1免疫染色仅限于CN神经元末端，而vGluT3免疫标记神经元体。这种情况在耳蜗腹侧核（VCN）聋后3天发生了变化，vGluT1免疫染色不再出现在大听神经末梢，而是在VCN神经元的体细胞中发现。在耳蜗背核（DCN）中，虽然vGluT1末端标记减少，但神经元体无标记。因此，外周兴奋性输入的缺失导致vGluT1和vGluT3在VCN神经元体中共定位。突触后谷氨酸能神经元可以通过逆行信号控制其突触前输入，这些结果表明在不同的兴奋性输入条件下，vGluTs可能在CN中发挥调节逆行信号的作用。采用qRT-PCR检测耳聋后3周CN神经元vGluT基因表达变化，vGluT1基因在CN腹侧和背侧表达显著升高，vGluT3基因在VCN中表达降低，在DCN中表达升高。(c) 2011年由Elsevier Ltd代表IBRO出版。
An immunocytochemical comparison of vGluT1 and vGluT3 in the cochlear nucleus (CN) of deafened versus normal hearing rats showed the first example of vGluT3 immunostaining in the dorsal and ventral CN and revealed temporal and spatial changes in vGluT1 localization in the CN after cochlear injury. In normal hearing rats vGluT1 immunostaining was restricted to terminals on CN neurons while vGluT3 immunolabeled the somata of the neurons. This changed in the ventral cochlear nucleus (VCN) 3 days following deafness, where vGluT1 immunostaining was no longer seen in large auditory nerve terminals but was instead found in somata of VCN neurons. In the dorsal cochlear nucleus (DCN), while vGluT1 labeling of terminals decreased, there was no labeling of neuronal somata. Therefore, loss of peripheral excitatory input results in co-localization of vGluT1 and vGluT3 in VCN neuronal somata. Postsynaptic glutamatergic neurons can use retrograde signaling to control their presynaptic inputs and these results suggest vGluTs could play a role in regulating retrograde signaling in the CN under different conditions of excitatory input. Changes in vGluT gene expression in CN neurons were found 3 weeks following deafness using qRT-PCR with significant increases in vGluT1 gene expression in both ventral and dorsal CN while vGluT3 gene expression decreased in VCN but increased in DCN. (c) 2011 Published by Elsevier Ltd on behalf of IBRO.