Electrical pulse – mediated enhanced delivery of silver nanoparticles into living suspension cells for surface enhanced Raman spectroscopy
Electrical pulse – mediated enhanced delivery of silver nanoparticles into living suspension cells for surface enhanced Raman spectroscopy
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DOI：
10.1002/lapl.201110125
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发表时间：
2012-03
影响因子：
1.7
通讯作者：
Juqiang Lin;Yun Yu;Buhong Li;Hao Huang;S. Lin;C. Li;Y. Su;S. Feng;Guannan Chen;
中科院分区：
文献类型：
--
作者：
Juqiang Lin;Yun Yu;Buhong Li;Hao Huang;S. Lin;C. Li;Y. Su;S. Feng;Guannan Chen;
电脉冲介导的增强银纳米粒子递送是悬浮细胞的细胞内表面增强拉曼光谱 (SERS) 测量的更好方法。基于电穿孔-SERS方法获得了稳定、高质量的活悬浮细胞SERS光谱，克服了电穿孔后银纳米粒子在细胞胞质中分布不均匀的缺点，并减少了银纳米粒子递送到不同细胞中的量差异。电穿孔参数包括三个持续时间分别为 1、5 和 5 ms 的 150 V (375 V/cm) 电脉冲。我们的结果表明，相当数量的银纳米颗粒可以快速递送到人早幼粒细胞白血病HL60细胞中，并获得满意的SERS光谱，同时处理后的细胞的活力得到高度维持（91.7％）。电穿孔-SERS 方法为将银纳米粒子递送到活悬浮细胞中提供了巨大的潜在方法，这对于广泛的生物医学应用非常有用，包括活细胞的实时细胞内 SERS 分析。
Electrical pulse-mediated enhanced silver nanoparticles delivery is a much better method for intracellular surface-enhanced Raman spectroscopy (SERS) measurements of suspension cells. Robust and high-quality SERS spectra of living suspension cells were obtained based on an electroporation-SERS method, which can overcomes the shortcoming of non-uniform distribution of silver nanoparticles localized in the cell cytoplasm after electroporation and reduces the amount variance of silver nanoparticles delivered into different cells. The electroporation parameters include three 150 V (375 V/cm) electric pulses of 1, 5, and 5 ms durations respectively. Our results indicate that considerable amount of silver nanoparticles can be rapidly delivered into the human promyelocytic leukemia HL60 cells, and the satisfied SERS spectra were obtained while the viability of the treated cells was highly maintained (91.7%). The electroporation-SERS method offers great potential approach in delivering silver nanoparticles into living suspension cells, which is useful for widely biomedical applications including the real-time intracellular SERS analysis of living cells.