Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance.
Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance.
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DOI：
10.1016/j.bjm.2016.10.024
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Shahpiri A
中科院分区：
文献类型：
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作者：
Ansarypour Z;Shahpiri A
金属硫蛋白是低分子量、富含半胱氨酸 (Cys) 的蛋白质超家族，被认为在防止金属毒性和氧化应激方面发挥重要作用。本研究的主要目的是探讨水稻金属硫蛋白异构体（OsMTI-1b）的异源表达对酿酒酵母对Cd2+、H2O2和乙醇胁迫耐受性的影响。将编码 OsMTI-1b 的基因克隆到 p426GPD 作为酵母表达载体。将新构建体转化至酿酒酵母感受态细胞。验证OsMTI-1b的异源表达后，新菌株和对照在胁迫条件下生长。与对照菌株相比，表达OsMTI-1b的转化酿酒酵母细胞在含有CdCl2的培养基中生长时表现出对Cd2+更高的耐受性并积累更多的Cd2+离子。此外，GST-OsMTI-1b 的异源表达赋予酿酒酵母细胞 H2O2 和乙醇耐受性。结果表明植物MT亚型的异源表达可以增强酿酒酵母对多种胁迫的耐受性。
Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing OsMTI-1b showed more tolerance to Cd2+ and accumulated more Cd2+ ions when they were grown in the medium containing CdCl2. In addition, the heterologous expression of GST-OsMTI-1b conferred H2O2 and ethanol tolerance to S. cerevisiae cells. The results indicate that heterologous expression of plant MT isoforms can enhance the tolerance of S. cerevisiae to multiple stresses.