High Concentration of Melatonin Regulates Leaf Development by Suppressing Cell Proliferation and Endoreduplication in Arabidopsis.
High Concentration of Melatonin Regulates Leaf Development by Suppressing Cell Proliferation and Endoreduplication in Arabidopsis.
复制标题
DOI：
10.3390/ijms18050991
复制
发表时间：
2017-05-05
影响因子：
5.6
通讯作者：
He C
中科院分区：
文献类型：
--
作者：
Wang Q;An B;Shi H;Luo H;He C
N-乙酰基-5-甲氧基色胺（Melatonin）是植物体内重要的信使，在调节生物节律、胁迫耐受、植物生长发育等方面发挥重要作用。一些研究表明外源褪黑激素处理对植物生长发育有阻滞作用。然而，褪黑激素在体内对植物叶片生长的调节作用及其机制尚不清楚。在这项研究中，我们发现，高浓度的褪黑激素抑制拟南芥叶片的生长，减少细胞的大小和细胞数量。对第5叶的进一步动力学分析表明，褪黑激素显著抑制细胞分裂速率。此外，流式细胞术分析表明，褪黑激素负调控叶片发育过程中的核内复制。因此，表达分析表明，褪黑素调节细胞周期和核糖体的关键基因的转录水平。综上所述，本研究表明，高浓度褪黑激素对拟南芥叶片的生长发育具有负调控作用，其机制可能是通过调节细胞核内复制、细胞周期和核糖体关键基因的转录水平。
N-acetyl-5-methoxytryptamine (Melatonin), as a crucial messenger in plants, functions in adjusting biological rhythms, stress tolerance, plant growth and development. Several studies have shown the retardation effect of exogenous melatonin treatment on plant growth and development. However, the in vivo role of melatonin in regulating plant leaf growth and the underlying mechanism are still unclear. In this study, we found that high concentration of melatonin suppressed leaf growth in Arabidopsis by reducing both cell size and cell number. Further kinetic analysis of the fifth leaves showed that melatonin remarkably inhibited cell division rate. Additionally, flow cytometic analysis indicated that melatonin negatively regulated endoreduplication during leaf development. Consistently, the expression analysis revealed that melatonin regulated the transcriptional levels of key genes of cell cycle and ribosome. Taken together, this study suggests that high concentration of melatonin negatively regulated the leaf growth and development in Arabidopsis, through modulation of endoreduplication and the transcripts of cell cycle and ribosomal key genes.