Modulation of heterochromatin protein 1 dynamics in primary mammalian cells
Modulation of heterochromatin protein 1 dynamics in primary mammalian cells
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DOI：
10.1126/science.1078694
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发表时间：
2003-01-31
期刊：
影响因子：
56.9
通讯作者：
Kioussis, D
中科院分区：
文献类型：
--
作者：
Festenstein, R;Pagakis, SN;Kioussis, D
异染色质蛋白 1 (HP1beta) 是浓缩 DNA 的关键成分，与基因沉默和着丝粒凝聚密切相关。异染色质被认为是一种静态结构，稳定核组织的关键方面并阻止转录因子的进入。我们在这里通过光漂白后的荧光恢复证明，绿色荧光蛋白-HP1β融合蛋白在离体静息小鼠T细胞的常染色质和异染色质中具有高度的迁移性。此外，T细胞激活大大增加了这种流动性，表明这一过程可能促进（异源）染色质重塑，并允许表观遗传修饰剂和转录因子接触到许多因此而被去抑制的基因。
Heterochromatin protein 1 (HP1beta), a key component of condensed DNA, is strongly implicated in gene silencing and centromeric cohesion. Heterochromatin has been considered a static structure, stabilizing crucial aspects of nuclear organization and prohibiting access to transcription factors. We demonstrate here, by fluorescence recovery after photobleaching, that a green fluorescent protein-HP1beta fusion protein is highly mobile within both the euchromatin and heterochromatin of ex vivo resting murine T cells. Moreover, T cell activation greatly increased this mobility, indicating that such a process may facilitate (hetero)chromatin remodeling and permit access of epigenetic modifiers and transcription factors to the many genes that are consequently derepressed.