A Vault-Encapsulated Enzyme Approach for Efficient Degradation and Detoxification of Bisphenol A and Its Analogues.
A Vault-Encapsulated Enzyme Approach for Efficient Degradation and Detoxification of Bisphenol A and Its Analogues.
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DOI：
10.1021/acssuschemeng.8b05432
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发表时间：
2019-02
影响因子：
8.4
通讯作者：
Meng Wang;Yichang Chen;V. Kickhoefer;L. Rome;P. Allard;Shaily Mahendra
中科院分区：
文献类型：
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作者：
Meng Wang;Yichang Chen;V. Kickhoefer;L. Rome;P. Allard;Shaily Mahendra
我们报告了一种有效的和环境可持续的水处理方法，使用封装在生物拱顶纳米颗粒的酶。锰过氧化物酶（MnP），其稳定性显着延长封装到拱顶，快速催化内分泌干扰化合物，包括双酚A（BPA），双酚F（BPF），双酚AP（BPAP）的生物转化。拱顶封装的MNP（vMNP）处理在较低的酶剂量下去除80-95%的每种测试的双酚（BP），而游离的天然MNP（nMNP）在24小时内仅导致19-36%的去除。vMNP和nMNP的处理导致产物种类和丰度的相当大的差异，这与所观察到的BPs的雌激素活性的变化是一致的。为了测试vMnP催化的转化是否产生有毒中间体，我们评估了BP毒性的生物标志，即破坏生殖过程的能力。在模式生物秀丽隐杆线虫中测得的vMnP处理样品的毒性对于所有三种BP均显著降低，包括BPA暴露的生殖指标，如生育力降低和生殖细胞死亡增加。总的来说，我们的研究结果表明，vMNP系统代表了一种有效和安全的方法，用于去除BP和承诺的拱顶封装定制酶的发展，用于处理其他目标的有机化合物在污染的沃茨。
We report an effective and environmentally sustainable water treatment approach using enzymes encapsulated in biogenic vault nanoparticles. Manganese peroxidase (MnP), whose stability was remarkably extended by encapsulating into vaults, rapidly catalyzed the biotransformation of endocrine-disrupting compounds, including bisphenol A (BPA), bisphenol F (BPF), and bisphenol AP (BPAP). The vault-encapsulated MnP (vMnP) treatment removed 80-95% of each of the tested bisphenols (BPs) at lower enzyme dosage, while free native MnP (nMnP) only resulted in a 19-36% removal, over a 24-h period. Treatment by vMnP and nMnP resulted in considerable disparities in product species and abundance, which were consistent with the observed changes in the estrogenic activities of BPs. To test if vMnP-catalyzed transformations generated toxic intermediates, we assessed biological hallmarks of BP toxicity, namely, the ability to disrupt reproductive processes. The toxicity of vMnP-treated samples, as measured in the model organism, Caenorhabditis elegans, was dramatically reduced for all three BPs, including the reproductive indicators of BPA exposure such as reduced fertility and increased germ cell death. Collectively, our results indicate that the vMnP system represents an efficient and safe approach for the removal of BPs and promise the development of vault-encapsulated customized enzymes for treating other targeted organic compounds in contaminated waters.