Nuclear receptor mRNA expression by HBV in human hepatoblastoma cell lines.
Nuclear receptor mRNA expression by HBV in human hepatoblastoma cell lines.
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DOI：
10.1016/j.canlet.2011.07.025
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发表时间：
2011-12
期刊：
影响因子：
9.7
通讯作者：
Shuang Wu;T. Kanda;F. Imazeki;Shingo Nakamoto;H. Shirasawa;O. Yokosuka
中科院分区：
文献类型：
--
作者：
Shuang Wu;T. Kanda;F. Imazeki;Shingo Nakamoto;H. Shirasawa;O. Yokosuka
最近的研究表明，核受体（NRs）在肝癌的发展。我们假设乙型肝炎B病毒（HBV）改变了NR和辅调节因子的表达，并通过SyBr实时RT-PCR比较了分泌完整HBV病毒粒子的HpeG2.2.15和HepG 2之间84个NR和相关基因的基因表达谱。绿色。与HepG2相比，HepG2.2.15中有40个（47.6%）基因上调2倍或更多，只有5个（5.9%）基因下调2倍或更多。这些结果表明，HBV影响核受体及其相关的信号转导，它们在病毒复制和HBV相关的肝癌发生中起重要作用。
Recent studies have implicated nuclear receptors (NRs) in the development of hepatocarcinogenesis. We assumed that hepatitis B virus (HBV) alters the expression of NRs and coregulators, and compared the gene expression profiling for 84 NRs and related genes between HpeG2.2.15, which secretes complete HBV virion, and HepG2 by real-time RT-PCR with SyBr green. Forty (47.6%) genes were upregulated 2-fold or greater, and only 5 (5.9%) were downregulated 2-fold or more, in HepG2.2.15 compared to HepG2. These results suggest that HBV affects NRs and their related signal transduction, and that they play important roles in viral replication and HBV-related hepatocarcinogenesis.