ANG II AT2 receptor modulates AT1 receptor-mediated descending vasa recta endothelial Ca2+ signaling.
ANG II AT2 receptor modulates AT1 receptor-mediated descending vasa recta endothelial Ca2+ signaling.
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ANG II AT2 受体调节 AT1 受体介导的降血管直肠内皮 Ca2 信号传导。
DOI：
10.1152/ajpheart.00317.2002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Pallone,ThomasL
中科院分区：
文献类型：
--
作者：
Rhinehart,Kristie;Handelsman,CoreyA;Silldorff,ErikP;Pallone,ThomasL
We tested whether the respective angiotensin type 1 (AT1) and 2 (AT2) receptor subtype antagonists losartan and PD-123319 could block the descending vasa recta (DVR) endothelial intracellular calcium concentration ([Ca2+]i) suppression induced by ANG II. ANG II partially reversed the increase in [Ca2+]igenerated by cyclopiazonic acid (CPA; 10−5M), acetylcholine (ACh; 10−5M), or bradykinin (BK; 10−7M). Losartan (10−5M) blocked that effect. When vessels were treated with ANG II before stimulation with BK and ACh, concomitant AT2receptor blockade with PD-123319 (10−8M) augmented the suppression of endothelial [Ca2+]iresponses. Similarly, preactivation with the AT2receptor agonist CGP-42112A (10−8M) prevented AT1receptor stimulation with ANG II + PD-123319 from suppressing endothelial [Ca2+]i. In contrast to endothelial [Ca2+]isuppression by ANG II, pericyte [Ca2+]iexhibited typical peak and plateau [Ca2+]iresponses that were blocked by losartan but not PD-123319. DVR vasoconstriction by ANG II was augmented when AT2receptors were blocked with PD-123319. Similarly, AT2receptor stimulation with CGP-42112A delayed the onset of ANG II-induced constriction. PD-123319 alone (10−5M) showed no AT1-like action to constrict microperfused DVR or increase pericyte [Ca2+]i. We conclude that ANG II suppression of endothelial [Ca2+]iand stimulation of pericyte [Ca2+]iis mediated by AT1or AT1-like receptors. Furthermore, AT2receptor activation opposes ANG II-induced endothelial [Ca2+]isuppression and abrogates ANG II-induced DVR vasoconstriction.
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DOI：
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