Development of the histaminergic neurons and expression of histidine decarboxylase mRNA in the zebrafish brain in the absence of all peripheral histaminergic systems
Development of the histaminergic neurons and expression of histidine decarboxylase mRNA in the zebrafish brain in the absence of all peripheral histaminergic systems
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DOI：
10.1046/j.1460-9568.1998.00394.x
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发表时间：
1998-12-01
影响因子：
3.4
通讯作者：
Panula, P
中科院分区：
文献类型：
--
作者：
Eriksson, KS;Peitsaro, N;Panula, P
用免疫细胞化学和层析方法研究了成年斑马鱼和发育斑马鱼的组胺储存神经系统。此外，还克隆了部分组氨酸脱羧酶基因，并用原位杂交技术对其表达进行了定位。组胺储存神经元仅在间脑室后隐窝周围的下丘脑尾部可见。除了小脑，几乎大脑的所有部位都至少含有一些组胺免疫反应纤维。上升投射以背端脑吻侧部分为主要目标。下降的突出在半圆形环部终止，中央灰色和下橄榄色。视神经顶盖的突出神经支配，这在其他鱼类中未见报道。原位杂交在与组胺免疫反应神经元解剖位置相同的细胞中给出了强烈的信号。在受精后约85小时，第一个组胺免疫反应神经元出现在下丘脑腹侧，90小时，免疫反应纤维终止于背端脑。在哺乳动物中描述的胚胎组胺生产在这个物种中是缺乏的。免疫细胞化学和色谱研究表明，组胺在斑马鱼身体的所有其他部位都不存在，除了下丘脑外，在鱼的任何其他部位都没有发现特异性杂交。因此，斑马鱼可能是一个非常有用的模型，用于大脑组胺能系统的体内药理学研究，因为该物种缺乏强大的组胺外周作用。
The histamine-storing neural system in adult and developing zebrafish (Danio rerio) was studied with immunocytochemical and chromatographical methods. Furthermore, the gene for histidine decarboxylase was partially cloned and its expression mapped with in situ hybridization. The histamine-storing neurons were only seen in the caudal hypothalamus, around the posterior recess of the diencephalic ventricle. Almost all parts of the brain, except the cerebellum, contained at least some histamine-immunoreactive fibres. The ascending projections had the rostral part of the dorsal telencephalon as a major target. Descending projections terminated in the torus semicircularis, central grey and inferior olive. A prominent innervation of the optic tectum, which has not been reported in other fish, was seen. The in situ hybridization gave a strong signal in cells with the same anatomical position as the histamine-immunoreactive neurons. The first histamine-immunoreactive neurons appeared in the ventral hypothalamus at about 85 h post-fertilization, and at 90 h, immunoreactive fibres terminated in the dorsal telencephalon. The embryonic histamine production described in mammals was lacking in this species. Both immunocytochemical and chromatographical studies indicated that histamine is absent in all other parts of the zebrafish body, and no specific hybridization was seen in any other part of the fish than the hypothalamus. The zebrafish could therefore be a very useful model for pharmacological in vivo studies of the histaminergic system of the brain, since the powerful peripheral actions of histamine should be lacking in this species.