SAMPLE STACKING OF AN EXTREMELY LARGE INJECTION VOLUME IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS
SAMPLE STACKING OF AN EXTREMELY LARGE INJECTION VOLUME IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS
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DOI：
10.1021/ac00033a015
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发表时间：
1992-05-01
影响因子：
7.4
通讯作者：
BURGI, DS
中科院分区：
文献类型：
--
作者：
CHIEN, RL;BURGI, DS
样品堆积是高效毛细管电泳（HPCE）中一种简单的柱上浓缩技术。然而，由于低浓度样品缓冲液引起的干扰，在常规样品堆积中可以加载到柱中的样品的量相当有限。本报告描述了一种将极大的样品体积堆积成窄带的技术。该技术基于这样的原理：样品缓冲液内离子的局部电泳速度比溶液的整体电渗透速度快得多。通过在加载样品后立即施加极性相反的高电压，可以在分离分析物之前去除样品缓冲液。由于从系统中去除了峰展宽机制，因此对于大的样品体积可以实现更好的分辨率。由于大量的样品可以加载到柱上，因此获得了数百倍的信号增强
Sample stacking is a simple on-column concentration technique in high-performance capillary electrophoresis (HPCE). However, the amount of sample which can be loaded into the column in conventional sample stacking is rather limited because of disturbances caused by the low-concentration sample buffer. This report describes a technique of stacking an extremely large sample volume into narrow bands. The technique is based on the principal that the local electrophoretic velocity of the ions inside the sample buffer is much faster than the bulk electroosmotic velocity of the solution By applying a high voltage with reversed polarity immediately after loading the sample, one can remove the sample buffer prior to separation of analytes. Since the peak-broadening mechanism is removed from the system, better resolution can be achieved for a large sample volume. Since large amounts of sample can be loaded onto the column, a factor of several hundreds in signal enhancement is obtained